ST1033 Anti-Shc Rabbit pAb

ST1033
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Pricing & Availability

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ST1033-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~46, ~55, and ~66 kDa isoforms of Shc in EGF-treated A431 cells.
      Catalogue NumberST1033
      Brand Family Calbiochem®
      References
      ReferencesHumtsoe, J.O., et al. 2003. EMBO J. 22, 1539.
      Wary, K.K, et al. 1988. Cell 94, 625
      Product Information
      FormLiquid
      Positive controlA431 cells stimulated with EGF
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Enzyme-Linked Immunosorbent Assay
      Immunocytochemistry
      Immunohistochemistry
      Application NotesELISA (1:1000-1:100,000)
      Immunofluorescence (20-50 µg/ml, indirect)
      Immunohistochemistry (20-50 µg/ml)
      Immunoprecipitation (1-4 µg/mg protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of the Sh2 domain of human Shc, fused to GST
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Shc Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1033

      References

      Reference overview
      Humtsoe, J.O., et al. 2003. EMBO J. 22, 1539.
      Wary, K.K, et al. 1988. Cell 94, 625
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2010 RFH
      ApplicationELISA (1:1000-1:100,000)
      Immunofluorescence (20-50 µg/ml, indirect)
      Immunohistochemistry (20-50 µg/ml)
      Immunoprecipitation (1-4 µg/mg protein)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~46, ~55, and ~66 kDa isoforms of Shc.
      BackgroundShc is an SH2-PTB domain adaptor protein that links various tyrosine kinases to Ras by recruiting the Grb2/SOS complex to the plasma membrane. Note that the ~66 kDa isoform is not typically expressed in hematapoetic cells.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of the Sh2 domain of human Shc, fused to GST
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlA431 cells stimulated with EGF
      FormLiquid
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHumtsoe, J.O., et al. 2003. EMBO J. 22, 1539.
      Wary, K.K, et al. 1988. Cell 94, 625