ST1617 Anti-SPG3A Mouse mAb (1B9)

ST1617
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, R M Monoclonal Antibody

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ST1617-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~50 kDa SPG3A protein in PC-12 and IMR32 cells.
      Catalogue NumberST1617
      Brand Family Calbiochem®
      SynonymsAnti-Atlastin GTPase 1
      Application Data
      Detection of rat SPG3A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SPG3A by immunoblotting. Sample: Whole cell lysate from IMR32 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.29.
      Positive controlPC-12 cells, IMR32 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human SPG3A, espressed as a GST fusion protein
      Clone1B9
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-SPG3A Mouse mAb (1B9) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-November-2010 RFH
      SynonymsAnti-Atlastin GTPase 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of rat SPG3A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SPG3A by immunoblotting. Sample: Whole cell lysate from IMR32 cells. Primary antibody: Anti-SPG3A Mouse mAb (1B9) (Cat. No. ST1617) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa SPG3A protein.
      BackgroundThe protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human SPG3A, espressed as a GST fusion protein
      Clone1B9
      IsotypeIgG₁
      Specieshuman, rat
      Positive controlPC-12 cells, IMR32 cells
      FormLiquid
      FormulationIn PBS, pH 7.29.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review