Product Information
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein
Clone1F12-1G7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-S100A4 Mouse mAb (1F12-1G7) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-S100 Calcium Binding Protein A4
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human S100A4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse S100A4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human S100A4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouae monoclonal antibody. Recognizes the ~10 kDa S100A4 protein.
BackgroundThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
HostMouse
ImmunogenFull-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein
Clone1F12-1G7
IsotypeIgG₁
Specieshuman, mouse
Positive controlHeLa cells, NIH3T3 cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies