NA18 Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19)

NA18
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ht, H M Monoclonal Antibody

Pricing & Availability

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NA18-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~34 kDa unphosphorylaed and the complexed (phosphorylated) subunit of replication protein A in HeLa and U293 cells and breast carcinoma tissue.
      Catalogue NumberNA18
      Brand Family Calbiochem®
      SynonymsAnti-RP-A
      References
      ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
      Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
      Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
      Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
      Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Positive controlHeLa or U293 cells or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (2.5-5 µg/ml, see comments)
      Application CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenreplication protein A purified from U293 cells
      ImmunogenHuman
      EpitopeWithin the p34 subunit of replication protein A
      CloneRPA34-19
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Hamster
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19) Certificates of Analysis

      TitleLot Number
      NA18

      References

      Reference overview
      Din, S., et al. 1990. Genes Dev. 4, 968.
      Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
      Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
      Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
      Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      SynonymsAnti-RP-A
      ApplicationImmunoblotting (5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (2.5-5 µg/ml, see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~34 kDa phosphorylated subunit and the nonphosphorylated RPA complex.
      BackgroundThe replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.
      HostMouse
      Immunogen speciesHuman
      Immunogenreplication protein A purified from U293 cells
      EpitopeWithin the p34 subunit of replication protein A
      CloneRPA34-19
      IsotypeIgG2a
      Specieshamster, human
      Positive controlHeLa or U293 cells or breast carcinoma tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
      Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
      Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
      Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
      Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.