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05-1000 Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtELISA, FC, IF, IH(P), WBMPurifiedMonoclonal Antibody
      Catalogue Number05-1000
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
      Background InformationThe identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).
      Product Information
      • Included Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is provided as a free positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC.
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.
      Quality LevelMQ100
      ApplicationDetect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunofluorescence

      Flow Cytometry

      Immunohistochemistry (Paraffin)

      Biological Information
      ImmunogenPhosphoserine coupled to KLH.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes serine-phosphorylated proteins from all species.
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the serine phosphorylated protein being detected.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.

      Western Blot Analysis:
      0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) SDS


      Safety Data Sheet (SDS) 

      Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) Certificates of Analysis

      TitleLot Number
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2137995 2137995
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2433477 2433477
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2023922 2023922
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 1964542 1964542
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2016023 2016023
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2201580 2201580
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2326373 2326373
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 2890779 2890779
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 3280139 3280139
      Anti-Phosphoserine, clone 4A4 (mouse monoclonal IgG1) - 3488917 3488917


      Reference overviewApplicationPub Med ID
      A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration.
      Cao, X; Kaneko, T; Li, JS; Liu, AD; Voss, C; Li, SS
      Nature communications  6  7721  2015

      Show Abstract
      26166433 26166433
      Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer.
      Cai, H; Katoh-Kurasawa, M; Muramoto, T; Santhanam, B; Long, Y; Li, L; Ueda, M; Iglesias, PA; Shaulsky, G; Devreotes, PN
      Science (New York, N.Y.)  343  1249531  2014

      Show Abstract
      Western Blotting24653039 24653039
      BAF complexes facilitate decatenation of DNA by topoisomerase IIα.
      Dykhuizen, EC; Hargreaves, DC; Miller, EL; Cui, K; Korshunov, A; Kool, M; Pfister, S; Cho, YJ; Zhao, K; Crabtree, GR
      Nature  497  624-7  2013

      Show Abstract
      23698369 23698369
      Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition.
      Zhang, S; Wang, X; Iqbal, S; Wang, Y; Osunkoya, AO; Chen, Z; Chen, Z; Shin, DM; Yuan, H; Wang, YA; Zhau, HE; Chung, LW; Ritenour, C; Kucuk, O; Wu, D
      The Journal of biological chemistry  288  1469-79  2013

      Show Abstract
      23188829 23188829
      Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures.
      Lu, Q; Wainwright, MS; Harris, VA; Aggarwal, S; Hou, Y; Rau, T; Poulsen, DJ; Black, SM
      Free radical biology & medicine  53  1139-51  2012

      Show Abstract
      22728269 22728269
      Estrous cycle variations in GABA(A) receptor phosphorylation enable rapid modulation by anabolic androgenic steroids in the medial preoptic area.
      Oberlander, JG; Porter, DM; Onakomaiya, MM; Penatti, CA; Vithlani, M; Moss, SJ; Clark, AS; Henderson, LP
      Neuroscience  226  397-410  2012

      Show Abstract
      22989919 22989919
      5-Aza-2'-deoxycytidine reactivates gene expression via degradation of pRb pocket proteins.
      Zheng, Z; Li, L; Liu, X; Wang, D; Tu, B; Wang, L; Wang, H; Zhu, WG
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  26  449-59  2012

      Show Abstract
      21990374 21990374
      Glycine receptor internalization by protein kinases activation.
      Miguel Ángel Velázquez-Flores,Rocío Salceda,Miguel Ángel Velázquez-Flores,Rocío Salceda
      Synapse (New York, N.Y.)  65  2011

      Show Abstract
      21656573 21656573
      Osmotic stress stimulates phosphorylation and cellular expression of heat shock proteins in rhesus macaque sperm.
      Cole JA, Meyers SA
      Journal of andrology  32  402-10. Epub 2010 Nov 18.  2011

      Show Abstract
      21088232 21088232
      CDK11p58 phosphorylation of PAK1 Ser174 promotes DLC2 binding and roles on cell cycle progression.
      Kong, Xiangfei, et al.
      J. Biochem., 146: 417-27 (2009)  2009

      Show Abstract
      19520772 19520772

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      05-1000X Anti-Phosphoserine Antibody, clone 4A4 Show Pricing & Availability

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      Life Science Research > Antibodies and Assays > Primary Antibodies