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05-577 Anti-PP2A Antibody, C subunit, demethylated, clone 4b7

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtICC, IP, WBMAscitesMonoclonal Antibody
      Catalogue Number05-577
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PP2A Antibody, C subunit, demethylated, clone 4b7
      Product Information
      HS Code3206 49 90
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Quality LevelMQ100
      ApplicationAnti-PP2A Antibody, C subunit, demethylated, clone 4b7 is an antibody against PP2A for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of human PP2A, C subunit
      Cloneclone 4b7
      Specificityunmethylated PP2A, C subunit
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
      Gene Symbol
      • PPP2CA
      • PP2CA
      • PP2A-alpha
      • PP2Ac
      • RP-C
      Purification MethodAscites
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
      COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
      SIZE: 309 amino acids; 35594 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
      PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
      SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Molecular Weight36kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      05-577 04053252339370


      Anti-PP2A Antibody, C subunit, demethylated, clone 4b7 SDS


      Safety Data Sheet (SDS) 

      Anti-PP2A Antibody, C subunit, demethylated, clone 4b7 Certificates of Analysis

      TitleLot Number
      Anti-PP2A, C subunit, demethylated, clone 4b7 3065561
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) - 2138013 2138013
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) - 2391087 2391087
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) 3154938
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) 2944580
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) 2857615
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) 3281733
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) - 1982666 1982666
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) - 2273967 2273967
      Anti-PP2A, C subunit, demethylated, clone 4b7 (diluted mouse ascites) - DAM1472192 DAM1472192


      Reference overviewApplicationPub Med ID
      NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink.
      Ulanovskaya, OA; Zuhl, AM; Cravatt, BF
      Nature chemical biology  9  300-6  2013

      Show Abstract
      Western Blotting23455543 23455543
      Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart.
      Qinghang Liu, Polly A Hofmann, Qinghang Liu, Polly A Hofmann
      American journal of physiology. Heart and circulatory physiology  283  H1314-21  2002

      Show Abstract
      12234781 12234781
      Methylation of the protein phosphatase 2A catalytic subunit is essential for association of Balpha regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen
      Yu, X. X., et al
      Mol Biol Cell, 12:185-99 (2001)  2001

      Immunoprecipitation11160832 11160832
      WD40 repeat proteins striatin and S/G(2) nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A.
      Moreno, C S, et al.
      J. Biol. Chem., 275: 5257-63 (2000)  2000

      Show Abstract
      10681496 10681496
      A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A.
      Ogris, E, et al.
      J. Biol. Chem., 274: 14382-91 (1999)  1999

      Show Abstract
      10318862 10318862

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      Life Science Research > Antibodies and Assays > Primary Antibodies