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05-545 Anti-PP2A Antibody, C subunit, clone 7A6

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Yeast (S. cerevisiae)IP, WBMCulture SupernatantMonoclonal Antibody
      Catalogue Number05-545
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PP2A Antibody, C subunit, clone 7A6
      Background InformationSerine/threonine-protein phosphatase 2A catalytic subunit beta isoform (UniProt: P62714; also known as EC:, PP2A-β) is encoded by the PPP2CB gene (Gene ID: 5516) in human. Phosphorylation and dephosphorylation of structural and regulatory proteins are major intracellular control mechanisms in eukaryotes. Protein phosphatase 2A is a major phosphoserine and phosphothreonine protein phosphatase present in all eukaryotic cells. It is involved in the regulation of several processes, including signaling cascades, cell cycle regulation, apoptosis, and development. It consists of a 36 kDa catalytic subunit (PP2A C), that is complexed with a 65 kDa regulatory subunit (PR65). This dimer associates with variable regulatory subunits of 55 kDa (PR55), 72 kDa (PR72), or 130 kDa (PR130). These various regulatory subunits affect substrate specificity and the subcellular distribution of PP2A. The PR72 unit contains a potential nuclear localization signal in its primary sequence, which may help PP2A to translocate to the nucleus. PP2A C has a highly conserved C terminus that plays an important role in regulating its phosphatase activity. In mammalian cells about 90% of PP2A C is present in the carboxymethylated state. This carboxyl methylation is believed to stimulate PP2A activity and promote PP2A heterotrimeric complex assembly. Dysregulation of PP2A methylation is reported to have important biological consequences. Reduced PP2A methylation has been linked to an increased risk of Alzheimer’s disease. Clone 7A6 is generated against a sequence from the C-terminal region of PPA2 C and it preferentially recognizes the non-methylated form of the enzyme and is not suitable for evaluating PP2A activity and holoenzyme composition. (Ref.: Frohner, IE., et al. (2020). Science Sig. 13(616); eaax6490).
      Product Information
      FormatCulture Supernatant
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
      PresentationCell culture supernatant containing 30% glycerol and 0.05% sodium azide as a preservative.
      Quality LevelMQ100
      ApplicationAnti-PP2A Antibody, C subunit, clone 7A6 preferentially recognizes the non-methylated form of the enzyme and is not suitable for evaluating PP2A activity and holoenzyme composition. (Ref.: Frohner, IE., et al. (2020). Science Sig. 13(616); eaaz6490).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide (C-bARRTPDYFL) corresponding to amino acids 302-309 of human PP2A catalytic subunit
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes the catalytic subunit of PP2A.
      Species Reactivity
      • Human
      • Mouse
      • Yeast (S. cerevisiae)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
      Gene Symbol
      • PPP2CA
      • PP2CA
      • PP2A-alpha
      • PP2Ac
      • RP-C
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
      COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
      SIZE: 309 amino acids; 35594 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
      PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
      SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Molecular Weight36 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      05-545 04053252281099


      Anti-PP2A Antibody, C subunit, clone 7A6 SDS


      Safety Data Sheet (SDS) 

      Anti-PP2A Antibody, C subunit, clone 7A6 Certificates of Analysis

      TitleLot Number
      Anti-PP2A, C subunit, clone 7A6 - 19189 19189
      Anti-PP2A, C subunit, clone 7A6 - 23721 23721
      Anti-PP2A, C subunit, clone 7A6 - 32190 32190
      Anti-PP2A, C subunit, clone 7A6 - DAM1487544 DAM1487544
      Anti-PP2A, C subunit, clone 7A6 - JBC1858523 JBC1858523
      Anti-PP2A, C-subunit (mouse ascites) - 2395691 2395691
      Anti-PP2A, C-subunit -2544317 2544317
      Anti-PP2A, C-subunit -2574847 2574847
      Anti-PP2A, C-subunit, clone 7A6 3022854
      Anti-PP2A, C-subunit, clone 7A6 2939509


      Reference overviewApplicationPub Med ID
      Antibodies recognizing the C terminus of PP2A catalytic subunit are unsuitable for evaluating PP2A activity and holoenzyme composition
      Ingrid E Frohner, Ingrid Mudrak, Stephanie Kronlachner, Stefan Schüchner, Egon Ogris<br />Affiliations<br />1. Center for Medical Biochemistry, Max Perutz Labs, Vienna BioCenter, Medical University of Vienna, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria.<br />2. Center for Medical Biochemistry, Max Perutz Labs, Vienna BioCenter, Medical University of Vienna, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria.
      Science Signaling  2020

      Show Abstract
      31992581 31992581
      The B55α regulatory subunit of protein phosphatase 2A mediates fibroblast growth factor-induced p107 dephosphorylation and growth arrest in chondrocytes.
      Kolupaeva, V; Daempfling, L; Basilico, C
      Molecular and cellular biology  33  2865-78  2013

      Show Abstract
      23716589 23716589
      Modeling initiation of Ewing sarcoma in human neural crest cells.
      von Levetzow, C; Jiang, X; Gwye, Y; von Levetzow, G; Hung, L; Cooper, A; Hsu, JH; Lawlor, ER
      PloS one  6  e19305  2011

      Show Abstract Full Text Article
      21559395 21559395
      The role of organic anion transporting polypeptides (OATPs/SLCOs) in the toxicity of different microcystin congeners in vitro: a comparison of primary human hepatocytes and OATP-transfected HEK293 cells.
      Fischer A, Hoeger SJ, Stemmer K, Feurstein DJ, Knobeloch D, Nussler A, Dietrich DR
      Toxicol Appl Pharmacol  245  9-20. Epub 2010 Feb 17.  2010

      Show Abstract
      20171238 20171238
      Regulation of L-type calcium channel and delayed rectifier potassium channel activity by p21-activated kinase-1 in guinea pig sinoatrial node pacemaker cells.
      Ke, Y; Lei, M; Collins, TP; Rakovic, S; Mattick, PA; Yamasaki, M; Brodie, MS; Terrar, DA; Solaro, RJ
      Circulation research  100  1317-27  2007

      Show Abstract
      Immunofluorescence17413045 17413045
      Loss of protein phosphatase 2A expression correlates with phosphorylation of DP-1 and reversal of dysplasia through differentiation in a conditional mouse model of cancer progression.
      Maddalena T Tilli, Shawnté L Hudgins, M Silvina Frech, Ewa D Halama, Jean-Pierre Renou, Priscilla A Furth
      Cancer research  63  7668-73  2003

      Show Abstract
      14633688 14633688
      Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A
      Yeong, F. M., et al
      Curr Biol, 13:2058-64 (2003)  2003

      Immunoprecipitation14653995 14653995
      Altering the holoenzyme composition and substrate specificity of protein phosphatase 2A
      Fellner, T., et al
      Methods Enzymol, 366:187-203 (2003)  2003

      Immunoprecipitation14674250 14674250
      ATM-dependent dissociation of B55 regulatory subunit from nuclear PP2A in response to ionizing radiation
      Guo, C. Y., et al
      J Biol Chem, 277:4839-44 (2002)  2002

      Immunoblotting (Western)11723136 11723136
      A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A.
      Ogris, E, et al.
      J. Biol. Chem., 274: 14382-91 (1999)  1999

      Show Abstract
      10318862 10318862


      What is the concentration of this antibody?We are not able to quantitate cultured supernatants.

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      Included Positive Control

      Catalog Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies