506107 Anti-P2X7 Receptor (576-595) Rabbit pAb

506107
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
R Rb Polyclonal Antibody

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506107-1SET
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      Description
      OverviewRecognizes the P2X7 receptor protein. Supplied with a control peptide.
      Catalogue Number506107
      Brand Family Calbiochem®
      Application Data
      Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
      References
      ReferencesCollo, G., et al. 1997. Neuropharmacology 36, 1277.
      Surprenant, A., et al. 1996. Science 272, 735.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:200-1:300, chemilimunescence)
      Application CommentsThe immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-P2X7 Receptor (576-595) Rabbit pAb Certificates of Analysis

      TitleLot Number
      506107

      References

      Reference overview
      Collo, G., et al. 1997. Neuropharmacology 36, 1277.
      Surprenant, A., et al. 1996. Science 272, 735.

      Citations

      Title
    • Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry 281, 4100-4108.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-August-2007 RFH
      ApplicationImmunoblotting (1:200-1:300, chemilimunescence)
      Application Data
      Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the P2X7 receptor protein. Supplied with control peptide (immunogen).
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
      IsotypeIgG
      Speciesrat
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
      Preservative≤0.1% sodium azide (antibody only)
      CommentsThe immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCollo, G., et al. 1997. Neuropharmacology 36, 1277.
      Surprenant, A., et al. 1996. Science 272, 735.
      Citation
    • Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry 281, 4100-4108.