IHCR1001-6 Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60

IHCR1001-6
6 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Ch, Ft, H, M, Po, R, Pm, Sal, Vrt IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberIHCR1001-6
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60
      Alternate Names
      • NeuN
      Background InformationNeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
      References
      Product Information
      FormatPurified
      PresentationLiquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% as preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60 detects level of Neuron-Specific Nuclear Protein & has been published & validated for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesAntibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

      Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

      Incubation: 30 minutes with IHC Select® Detection Kits.

      NeuN, has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1001-6.
      Biological Information
      ImmunogenPurified cell nuclei from mouse brain.
      CloneA60
      HostMouse
      Specificityneuron-specific protein NeuN
      Species Reactivity
      • Avian
      • Chicken
      • Ferret
      • Human
      • Mouse
      • Pig
      • Rat
      • Primate
      • Salamander
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C. Refer to vial for expiration dating.
      Packaging Information
      Material Size6 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60 Certificates of Analysis

      TitleLot Number
      CHEMICON IHC SELECT RESEARCH - 32443943244394
      CHEMICON IHC SELECT RESEARCH -27502852750285
      CHEMICON IHC SELECT RESEARCH PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN - 32404393240439
      CHEMICON IHC SELECT RESEARCH#210; - 22049042204904
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN - 20592782059278
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 19952361995236
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 20059492005949
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 20362772036277
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 21105622110562
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 21926942192694

      References

      Reference overviewPub Med ID
      Lateral Connectivity in the Olfactory Bulb is Sparse and Segregated.
      Kim, DH; Phillips, ME; Chang, AY; Patel, HK; Nguyen, KT; Willhite, DC
      Frontiers in neural circuits  5  5  2011

      Show Abstract Full Text Article
      21559072 21559072

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      Life Science Research > Antibodies and Assays > Primary Antibodies