ABN78A4 Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate

ABN78A4
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R ICC, IHC Rb AlexaFluor®488 Polyclonal Antibody
      Description
      Catalogue NumberABN78A4
      DescriptionAnti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • NEUronal Nuclei
      • Neuronal Nuclei
      Background InformationABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Adult mouse brain tissue
      PresentationPurified rabbit polyclonal conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect NeuN (rabbit) using this Anti-NeuN (rabbit) Antibody, Alexa Fluor 488 Conjugate validated for use in IC, IH.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry analysis: A 1:100 dilution from a representative lot detected NeuN in rat E18 primary cortex cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse NeuN.
      EpitopeN-terminus
      HostRabbit
      SpecificityThis antibody recognizes NeuN at the N-terminus.
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NeuN
      Purification MethodAffinity Purfied
      Molecular WeightThe unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa. An uncharacterized band appears at ~62 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in adult mouse brain tissue.

      Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-NeuN (rabbit polyclonal) -25142182514218
      Anti-NeuN (rabbit polyclonal), Alexa Fluor® 488 Conjugate3074335
      Anti-NeuN (rabbit polyclonal), Alexa Fluor® 488 Conjugate - 24205362420536
      Anti-NeuN (rabbit polyclonal), Alexa Fluor® 488 Conjugate - 24524592452459
      Anti-NeuN (rabbit polyclonal), -25948672594867
      Anti-NeuN (rabbit polyclonal), -26162042616204
      Anti-NeuN (rabbit polyclonal), -26595962659596
      Anti-NeuN (rabbit polyclonal), -26861192686119
      Anti-NeuN (rabbit polyclonal), -26914542691454
      Anti-NeuN (rabbit polyclonal), Alexa Fluor#174; 488 Conjugate - 19882931988293

      References

      Reference overviewPub Med ID
      Estrogen receptor alpha deficiency protects against development of cognitive impairment in murine lupus.
      Cunningham, MA; Wirth, JR; Freeman, LR; Boger, HA; Granholm, AC; Gilkeson, GS
      Journal of neuroinflammation  11  171  2014

      Show Abstract
      25510908 25510908
      Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress.
      Gárate, I; García-Bueno, B; Madrigal, JL; Caso, JR; Alou, L; Gómez-Lus, ML; Leza, JC
      Journal of neuroinflammation  11  8  2014

      Show Abstract
      24410883 24410883