ABN78 Anti-NeuN Antibody (rabbit)

ABN78
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H ICC, IHC, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABN78
      DescriptionAnti-NeuN Antibody (rabbit)
      Alternate Names
      • RNA binding protein fox-1 homolog 3
      • Fox-1 homolog C
      • Fox-3
      • Hexaribonucleotide-binding protein 3
      • NeuN
      • Neuronal nuclei
      Background InformationRNA binding protein fox-1 homolog 3 (UniProt Q8BIF2; also known as Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse brain nuclear extract
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NeuN (rabbit polyclonal), Cat. No. ABN78, is rabbit polyclonal antibody that detect NeuN and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected NeuN in Mouse Brain Nuclear Extract.

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NeuN in human cerebral cortex and mouse hippocampus tissue sections.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in E18 neuronal cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to first 97 amino acids from the N-terminal region of murine NeuN.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects NeuN. It targets an epitope within first 97 amino acids from the N-terminal region.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Snail based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Rbfox3
      • D11Bwg0517e
      • Hrnbp3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~48/42 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse E16 Brain lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected NeuN in Mouse E16 Brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NeuN Antibody (rabbit) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NeuN Antibody (rabbit) Certificates of Analysis

      TitleLot Number
      Anti-NeuN (rabbit polyclonal)3065573
      Anti-NeuN (rabbit polyclonal) -31335503133550
      Anti-NeuN (rabbit polyclonal) - 21400862140086
      Anti-NeuN (rabbit polyclonal) - 23912102391210
      Anti-NeuN (rabbit polyclonal) - 24386872438687
      Anti-NeuN (rabbit polyclonal) - 24590792459079
      Anti-NeuN (rabbit polyclonal) - 19495971949597
      Anti-NeuN (rabbit polyclonal) - 19792711979271
      Anti-NeuN (rabbit polyclonal) - 20008252000825
      Anti-NeuN (rabbit polyclonal) - 20111512011151

      References

      Reference overviewApplicationPub Med ID
      Expression of progerin in aging mouse brains reveals structural nuclear abnormalities without detectible significant alterations in gene expression, hippocampal stem cells or behavior.
      Baek, JH; Schmidt, E; Viceconte, N; Strandgren, C; Pernold, K; Richard, TJ; Van Leeuwen, FW; Dantuma, NP; Damberg, P; Hultenby, K; Ulfhake, B; Mugnaini, E; Rozell, B; Eriksson, M
      Human molecular genetics  24  1305-21  2015

      Show Abstract
      25343989 25343989
      Arginase 1+ microglia reduce Aβ plaque deposition during IL-1β-dependent neuroinflammation.
      Cherry, JD; Olschowka, JA; O'Banion, MK
      Journal of neuroinflammation  12  203  2015

      Show Abstract
      26538310 26538310
      FTY720 attenuates excitotoxicity and neuroinflammation.
      Cipriani, R; Chara, JC; Rodríguez-Antigüedad, A; Matute, C
      Journal of neuroinflammation  12  86  2015

      Show Abstract
      25953296 25953296
      Cortical neurons are a prominent source of the proinflammatory cytokine osteopontin in HIV-associated neurocognitive disorders.
      Silva, K; Hope-Lucas, C; White, T; Hairston, TK; Rameau, T; Brown, A
      Journal of neurovirology  21  174-85  2015

      Show Abstract
      25636782 25636782
      Characterization of HTT inclusion size, location, and timing in the zQ175 mouse model of Huntington's disease: an in vivo high-content imaging study.
      Carty, N; Berson, N; Tillack, K; Thiede, C; Scholz, D; Kottig, K; Sedaghat, Y; Gabrysiak, C; Yohrling, G; von der Kammer, H; Ebneth, A; Mack, V; Munoz-Sanjuan, I; Kwak, S
      PloS one  10  e0123527  2015

      Show Abstract
      25859666 25859666
      Adversity in childhood and depression: linked through SIRT1.
      Lo Iacono, L; Visco-Comandini, F; Valzania, A; Viscomi, MT; Coviello, M; Giampà, A; Roscini, L; Bisicchia, E; Siracusano, A; Troisi, A; Puglisi-Allegra, S; Carola, V
      Translational psychiatry  5  e629  2015

      Show Abstract
      26327687 26327687
      Nigral proteasome inhibition in mice leads to motor and non-motor deficits and increased expression of Ser129 phosphorylated α-synuclein.
      Bentea, E; Van der Perren, A; Van Liefferinge, J; El Arfani, A; Albertini, G; Demuyser, T; Merckx, E; Michotte, Y; Smolders, I; Baekelandt, V; Massie, A
      Frontiers in behavioral neuroscience  9  68  2015

      Show Abstract
      25873870 25873870
      Frontotemporal dementia caused by CHMP2B mutation is characterised by neuronal lysosomal storage pathology.
      Clayton, EL; Mizielinska, S; Edgar, JR; Nielsen, TT; Marshall, S; Norona, FE; Robbins, M; Damirji, H; Holm, IE; Johannsen, P; Nielsen, JE; Asante, EA; Collinge, J; , ; Isaacs, AM
      Acta neuropathologica  130  511-23  2015

      Show Abstract
      26358247 26358247
      Direct neuronal glucose uptake Heralds activity-dependent increases in cerebral metabolism.
      Lundgaard, I; Li, B; Xie, L; Kang, H; Sanggaard, S; Haswell, JD; Sun, W; Goldman, S; Blekot, S; Nielsen, M; Takano, T; Deane, R; Nedergaard, M
      Nature communications  6  6807  2015

      Show Abstract
      25904018 25904018
      Distribution of GABAergic cells in the inferior colliculus that project to the thalamus.
      Mellott, JG; Foster, NL; Nakamoto, KT; Motts, SD; Schofield, BR
      Frontiers in neuroanatomy  8  17  2014

      Show Abstract
      24744703 24744703