MAB377B Anti-NeuN Antibody, clone A60, biotin conjugated

MAB377B
500 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, Ft, Ch, Sal ICC, IHC, IH(P), WB M Biotin Monoclonal Antibody
      Description
      Catalogue NumberMAB377B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NeuN Antibody, clone A60, biotin conjugated
      Alternate Names
      • Neuron-Specific Nuclear Protein
      Background InformationNeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
      References
      Product Information
      FormatBiotin
      Control
      • Brain tissue, most neuronal cell types throughout the adult nervous system
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.01 M PBS pH 7.1, 0.1% sodium azide with 15 mg/mL BSA as a stabilizer.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-NeuN Antibody, clone A60, biotin conjugated is validated for use in IC, IH, IH(P), WB for the detection of NeuN.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry Analysis:
      1:10-1:500 dilution of a previous lot was used. Neurons in culture should be permeablized with 0.1% triton X-100. All primary antibody dilutions should be performed with simple solutions containing only buffer and primary antibody without excess protein blocks or detergents.

      For dual labeling studies using mouse monoclonals, antibody incubations should be sequential with MAB377B last. First mouse monoclonal antibody should be first detected with anti-mouse secondary prior to incubating with MAB377B. Excess anti-mouse IgG may be blocked by incubating with 1% mouse serum prior to MAB377B incubation. Detection of biotinylated NeuN monoclonal is via streptavidin. In some cases in may be necessary to pretreat the tissue with avidin to block excess biotin prior to immunohistochemisty (Wood and Warnke, 1981).

      Immunohistochemistry:
      1:200-1:2,000. The antibody works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. The antibody works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully (Sarnat, 1998).

      Western Blotting Analysis:
      A previous lot of this antibody was used on western blot. Recognizes 2-3 bands in the 46-48 kDa range and possibly another band at approximately 66 kDa.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified cell nuclei from mouse brain
      CloneA60
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityVertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). MAB377B reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377B include Purkinje, mitral and photoreceptor cells. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Ferret
      • Chicken
      • Salamander
      Species Reactivity NoteRat and mouse. It is expected that the biotin conjugated antibody will also react with human, ferret, chick and salamander.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • NeuN
      • A59
      Purification MethodProtein A Purfied
      Molecular Weight46-48 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry on brain tissue.

      Immunohistochemistry(paraffin) Analysis:
      NeuN (cat. # MAB377B) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Note that there is no signal detected in the nucleus of Purkinje cells.
      Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NeuN Antibody, clone A60, biotin conjugated Certificates of Analysis

      TitleLot Number
      Anti-NeuN, clone A60, biotin - 32323753232375
      Anti-NeuN, clone A60, biotin conjugated3053791
      Anti-NeuN, clone A60, biotin conjugated - 23793392379339
      Anti-NeuN, clone A60, biotin conjugated - 24468652446865
      Anti-NeuN, clone A60, biotin conjugated - 22341522234152
      Anti-NeuN, clone A60, biotin conjugated - 25103262510326
      Anti-NeuN, clone A60, biotin conjugated - 31601263160126
      Anti-NeuN, clone A60, biotin conjugated - 32609253260925
      Anti-NeuN, clone A60, biotin conjugated - 34369883436988
      Anti-NeuN, clone A60, biotin conjugated - LV1506371LV1506371

      References

      Reference overviewApplicationSpeciesPub Med ID
      Context-induced reinstatement of methamphetamine seeking is associated with unique molecular alterations in Fos-expressing dorsolateral striatum neurons.
      Rubio, FJ; Liu, QR; Li, X; Cruz, FC; Leão, RM; Warren, BL; Kambhampati, S; Babin, KR; McPherson, KB; Cimbro, R; Bossert, JM; Shaham, Y; Hope, BT
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  5625-39  2015

      Show Abstract
      25855177 25855177
      Oxidation and nitration in dopaminergic areas of the prefrontal cortex from patients with bipolar disorder and schizophrenia.
      Kim, HK; Andreazza, AC; Yeung, PY; Isaacs-Trepanier, C; Young, LT
      Journal of psychiatry & neuroscience : JPN  39  276-85  2014

      Show Abstract
      Immunohistochemistry24485387 24485387
      Dorsal raphe neuroinflammation promotes dramatic behavioral stress dysregulation.
      Howerton, AR; Roland, AV; Bale, TL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  7113-23  2014

      Show Abstract
      24849347 24849347
      Autophagy-lysosome pathway associated neuropathology and axonal degeneration in the brains of alpha-galactosidase A-deficient mice.
      Nelson, MP; Tse, TE; O'Quinn, DB; Percival, SM; Jaimes, EA; Warnock, DG; Shacka, JJ
      Acta neuropathologica communications  2  20  2014

      Show Abstract
      24529306 24529306
      Differential contribution of TRPM4 and TRPM5 nonselective cation channels to the slow afterdepolarization in mouse prefrontal cortex neurons.
      Lei, YT; Thuault, SJ; Launay, P; Margolskee, RF; Kandel, ER; Siegelbaum, SA
      Frontiers in cellular neuroscience  8  267  2014

      Show Abstract
      25237295 25237295
      Detection of molecular alterations in methamphetamine-activated Fos-expressing neurons from a single rat dorsal striatum using fluorescence-activated cell sorting (FACS).
      Liu, QR; Rubio, FJ; Bossert, JM; Marchant, NJ; Fanous, S; Hou, X; Shaham, Y; Hope, BT
      Journal of neurochemistry  128  173-85  2014

      Show Abstract
      23895375 23895375
      Evaluating the role of neuronal nitric oxide synthase-containing striatal interneurons in methamphetamine-induced dopamine neurotoxicity.
      Fricks-Gleason, AN; Keefe, KA
      Neurotoxicity research  24  288-97  2013

      Show Abstract
      23575992 23575992
      Unique gene alterations are induced in FACS-purified Fos-positive neurons activated during cue-induced relapse to heroin seeking.
      Fanous, S; Guez-Barber, DH; Goldart, EM; Schrama, R; Theberge, FR; Shaham, Y; Hope, BT
      Journal of neurochemistry  124  100-8  2013

      Show Abstract
      23113797 23113797
      A rapid fluorescent method to quantify neuronal loss after experimental intracerebral hemorrhage.
      Chen-Roetling, J; Lu, X; Regan, KA; Regan, RF
      Journal of neuroscience methods  216  128-36  2013

      Show Abstract
      23583700 23583700
      Analysis of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain.
      Aschauer, DF; Kreuz, S; Rumpel, S
      PloS one  8  e76310  2013

      Show Abstract
      24086725 24086725

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      Life Science Research > Antibodies and Assays > Primary Antibodies