MAB377 Anti-NeuN Antibody, clone A60

MAB377
500 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Ch, Ft, H, M, Po, R, Sal FC, ICC, IF, IHC, IH(P), IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB377
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NeuN Antibody, clone A60
      Alternate Names
      • Neuron-Specific Nuclear Protein
      • Neuna60
      • A60
      Background InformationNeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
      References
      Product Information
      FormatPurified
      Control
      • Positive control -Brain Tissue. Negative control - Any non neuronal tissue eg Fibroblasts
      PresentationPurified mouse immunoglobulin IgG1 liquid in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NeuN Antibody, clone A60 detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A previous lot of this antibody recognized 2-3 bands in the 46-48 kDa range and possibly another band at approximately 66 kDa.

      Immunocytochemistry:
      1:10-1:100 dilution from a previous lot was used. Neurons in culture should be permeablized with 0.1% triton X-100. All primary antibody dilutions should be performed with simple solutions containing only buffer and primary antibody without excess protein blocks or detergents.

      Immunohistochemistry:
      1:100-1:1,000. The antibody works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. The antibody works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully (Sarnat, 1998).

      Immunohistochemistry(paraffin) Analysis: A previous lot was used for IH(P).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified cell nuclei from mouse brain
      CloneA60
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMILLIPORE's exclusive monoclonal antibody to vertebrate neuron-specific nuclear protein called NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377 include Purkinje, mitral and photoreceptor cells. The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Chicken
      • Ferret
      • Human
      • Mouse
      • Pig
      • Rat
      • Salamander
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • NeuN
      • A58
      Purification MethodProtein A Purfied
      Molecular Weight46/48 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry on brain tissue.

      Immunohistochemistry(paraffin) Analysis:
      NeuN (cat. # MAB377) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Note that there is no signal detected in the nucleus of Purkinje cells.
      Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NeuN Antibody, clone A60 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NeuN Antibody, clone A60 Certificates of Analysis

      TitleLot Number
      Anti-NeuN, clone A603075598
      Anti-NeuN, clone A60 - 21180332118033
      Anti-NeuN, clone A60 - 23756082375608
      Anti-NeuN, clone A60 - 23922832392283
      Anti-NeuN, clone A60 - 24245072424507
      Anti-NeuN, clone A60 - 24286712428671
      Anti-NeuN, clone A60 - 24532492453249
      Anti-NeuN, clone A60 - 19912631991263
      Anti-NeuN, clone A60 - 20623132062313
      Anti-NeuN, clone A60 - 20747652074765

      References

      Reference overviewApplicationSpeciesPub Med ID
      Effects of sleep and wake on astrocytes: clues from molecular and ultrastructural studies.
      Bellesi, M; de Vivo, L; Tononi, G; Cirelli, C
      BMC biology  13  66  2015

      Show Abstract
      26303010 26303010
      Subset of early radial glial progenitors that contribute to the development of callosal neurons is absent from avian brain.
      García-Moreno, F; Molnár, Z
      Proceedings of the National Academy of Sciences of the United States of America  112  E5058-67  2015

      Show Abstract
      26305942 26305942
      Attenuation of Cerebral Ischemic Injury in Smad1 Deficient Mice.
      Wong, JK; Chen, L; Huang, Y; Sehba, FA; Friedel, RH; Zou, H
      PloS one  10  e0136967  2015

      Show Abstract
      26317208 26317208
      Effects of Mild Chronic Intermittent Cold Exposure on Rat Organs.
      Wang, X; Che, H; Zhang, W; Wang, J; Ke, T; Cao, R; Meng, S; Li, D; Weiming, O; Chen, J; Luo, W
      International journal of biological sciences  11  1171-80  2015

      Show Abstract
      26327811 26327811
      Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain.
      Wang, G; Yang, H; Yan, S; Wang, CE; Liu, X; Zhao, B; Ouyang, Z; Yin, P; Liu, Z; Zhao, Y; Liu, T; Fan, N; Guo, L; Li, S; Li, XJ; Lai, L
      Molecular neurodegeneration  10  42  2015

      Show Abstract
      26334913 26334913
      Behavioral experience induces zif268 expression in mature granule cells but suppresses its expression in immature granule cells.
      Huckleberry, KA; Kane, GA; Mathis, RJ; Cook, SG; Clutton, JE; Drew, MR
      Frontiers in systems neuroscience  9  118  2015

      Show Abstract
      26347620 26347620
      Breadth of tuning in taste afferent neurons varies with stimulus strength.
      Wu, A; Dvoryanchikov, G; Pereira, E; Chaudhari, N; Roper, SD
      Nature communications  6  8171  2015

      Show Abstract
      26373451 26373451
      Localization of reelin signaling pathway components in murine midbrain and striatum.
      Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
      Cell and tissue research  359  393-407  2015

      Show Abstract
      Mouse25418135 25418135
      Vibrissa Self-Motion and Touch Are Reliably Encoded along the Same Somatosensory Pathway from Brainstem through Thalamus.
      Moore, JD; Mercer Lindsay, N; Deschênes, M; Kleinfeld, D
      PLoS biology  13  e1002253  2015

      Show Abstract
      26393890 26393890
      Forebrain microglia from wild-type but not adult 5xFAD mice prevent amyloid-β plaque formation in organotypic hippocampal slice cultures.
      Hellwig, S; Masuch, A; Nestel, S; Katzmarski, N; Meyer-Luehmann, M; Biber, K
      Scientific reports  5  14624  2015

      Show Abstract
      26416689 26416689

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies