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AB3081 Anti-Na+/H+ Exchanger-1 Antibody

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AB3081
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3081
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Na+/H+ Exchanger-1 Antibody
      Alternate Names
      • NHE1
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Kidney tissue (basolateral membrane)
      PresentationAffinity Purified immunoglobulin. Liquid in PBS and 0.1% BSA .
      Quality LevelMQ100
      Applications
      ApplicationDetect Na+/H+ Exchanger-1 using this Anti-Na+/H+ Exchanger-1 Antibody validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using Chemiluminescence technique.



      ELISA: 1:100,000 using 50-100 ng control peptide per well.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA 22 aa peptide within the cytoplasmic, C-terminal domain of rat NHE1, coupled to KLH.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Na+/H+ Exchanger 1 (NHE1). The immunogen peptide shows no significant homology with other NHE isoforms (NHE2-5).
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC9A1
      • APNH
      • +H
      • APNH1
      • FLJ42224
      • NHE-1
      • Na
      • NHE1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19634 # Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
      SIZE: 815 amino acids; 90763 Da
      SUBUNIT: Interacts with tescalcin, CHP and CHP2.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Kidney and intestine.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. & O-glycosylated.
      SIMILARITY: SwissProt: P19634 ## Belongs to the Na(+)/H(+) exchanger (TC 2.A.36.5.1) family.
      MISCELLANEOUS: Inhibited by amiloride and 5-amino-substituted derivatives and activated in a cooperative fashion by intracellular H(+). In quiescent cells upon growth factor stimulation, the apparent affinity for internal H(+) is increased, resulting in a persistent rise in cytoplasmic pH.
      Molecular Weight91 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB3081 04053252624803

      Documentation

      Anti-Na+/H+ Exchanger-1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Na+/H+ Exchanger-1 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] 3072334
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] POLYCLONAL ANTIBODY - 2119998 2119998
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] POLYCLONAL ANTIBODY - 2145054 2145054
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] - 3224602 3224602
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] - 3384086 3384086
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] - 3587290 3587290
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] - 4087562 4087562
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] -2761662 2761662
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] POLYCLONAL ANTIBODY 3122978
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] POLYCLONAL ANTIBODY 2900224

      References

      Reference overviewApplicationPub Med ID
      Angiotensin II-mediated biphasic regulation of proximal tubular Na+/H+ exchanger 3 is impaired during oxidative stress.
      Banday, AA; Lokhandwala, MF
      American journal of physiology. Renal physiology  301  F364-70  2011

      Show Abstract
      Western Blotting21593187 21593187
      Malignant gliomas display altered plasma membrane potential and pH regulation--interaction with Tc-99m-MIBI and Tc-99m-Tetrofosmin uptakes.
      N Perek, D Denoyer, F Dubois, F Koumanov
      General physiology and biophysics  21  381-404  2002

      Show Abstract
      12693711 12693711
      NHE1, NHE2, and NHE3 contribute to regulation of intracellular pH in murine duodenal epithelial cells.
      J Praetorius, D Andreasen, B L Jensen, M A Ainsworth, U G Friis, T Johansen
      American journal of physiology. Gastrointestinal and liver physiology  278  G197-206  2000

      Show Abstract
      10666043 10666043
      Mammalian Na+/H+ exchanger gene family: structure and function studies.
      Yun, C H, et al.
      Am. J. Physiol., 269: G1-11 (1995)  1995

      7631785 7631785
      Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins.
      Orlowski, J, et al.
      J. Biol. Chem., 267: 9331-9 (1992)  1992

      1577762 1577762

      Data Sheet

      Title
      RABBIT NA+/H+ EXCHANGER-1 [NHE1] POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies