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ST1699 Anti-NDE1 Mouse mAb (2G11-1C11)

ST1699
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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ST1699-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~40-45 kDa NDE1 protein in HeLa cells.
      Catalogue NumberST1699
      Brand Family Calbiochem®
      SynonymsAnti-nudE, Anti-Nuclear Distribution Gene E Homolog1
      Application Data
      Detection of human NDE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human NDE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesBradshaw, N.J., et al. 2009. Neurosci. Lett. 449, 228.
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see coments)
      Immunoblotting (1-4 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
      Clone2G11-1C11
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NDE1 Mouse mAb (2G11-1C11) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Bradshaw, N.J., et al. 2009. Neurosci. Lett. 449, 228.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-nudE, Anti-Nuclear Distribution Gene E Homolog1
      ApplicationELISA (see coments)
      Immunoblotting (1-4 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human NDE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human NDE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDE1 protein.
      BackgroundThis gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Alternative splicing results in multiple transcript variants.
      HostMouse
      ImmunogenFull-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
      Clone2G11-1C11
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesBradshaw, N.J., et al. 2009. Neurosci. Lett. 449, 228.