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PC342 Anti-MMP-2 (Ab-7) Rabbit pAb

PC342
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M Rb Polyclonal Antibody

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PC342-100UG
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      Plastic ampoule 100 μg
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      Description
      Overview

      This product has been discontinued.



      Recognizes the ~72 kDa latent form of the MMP-2 protein. Cross-reacts with recombinant MMP-9 at high concentrations but does not cross-react with native MMP-9.

      Catalogue NumberPC342
      Brand Family Calbiochem®
      SynonymsAnti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2, Anti-Gelatinase A
      References
      ReferencesNikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427.
      Soini, Y., et al. 1994. J. Histochem. And Cytochemistry 42, 945.
      Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191.
      Fridman, R., et al. 1992. J. Biol. Chem. 267, 15398.
      Margulies, I.M., et al. 1992. Cancer Epidemiology, Biomarkers and Prevention 1, 467.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
      Product Information
      FormLiquid
      FormulationIn 1X PBS, 0.2% BSA.
      Positive controlConditioned medium from HT-1080 cells or recombinant MMP-2
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Frozen Sections
      Application NotesFrozen Sections (not recommended
      Immunoblotting (2 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Application CommentsThis antibody will cross-react with recombinant human MMP-9 at high concentrations of protein. No cross-reactivity seen with native MMP-9. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human pro-MMP-2
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MMP-2 (Ab-7) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MMP-2 (Ab-7) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC342

      References

      Reference overview
      Nikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427.
      Soini, Y., et al. 1994. J. Histochem. And Cytochemistry 42, 945.
      Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191.
      Fridman, R., et al. 1992. J. Biol. Chem. 267, 15398.
      Margulies, I.M., et al. 1992. Cancer Epidemiology, Biomarkers and Prevention 1, 467.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      SynonymsAnti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2, Anti-Gelatinase A
      ApplicationFrozen Sections (not recommended
      Immunoblotting (2 µg/ml)
      Immunoprecipitation (1 µg/sample)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~72 kDa latent form of MMP-2.
      BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-2 (Gelatinase A, 72 kDa gelatinase, type IV collagenase, TBE-1) is synthesized as a 631 amino acid proenzyme (72 kDa zymogen) which is proteolytically processed at the first 80 amino acids to the 66 kDa active form. MMP-2, along with its most closely related member of the MMP family, MMP-9, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that MMP-2 may be a useful marker for the diagnosis or prognosis of cancer.
      HostRabbit
      Immunogen speciesHuman
      Immunogenrecombinant, human pro-MMP-2
      IsotypeIgG
      Specieshuman, mouse
      Positive controlConditioned medium from HT-1080 cells or recombinant MMP-2
      FormLiquid
      FormulationIn 1X PBS, 0.2% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody will cross-react with recombinant human MMP-9 at high concentrations of protein. No cross-reactivity seen with native MMP-9. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesNikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427.
      Soini, Y., et al. 1994. J. Histochem. And Cytochemistry 42, 945.
      Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191.
      Fridman, R., et al. 1992. J. Biol. Chem. 267, 15398.
      Margulies, I.M., et al. 1992. Cancer Epidemiology, Biomarkers and Prevention 1, 467.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.