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07-2054 Anti-MBD1 Antibody, CT

07-2054
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISARbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2054
      DescriptionAnti-MBD1 Antibody, CT
      Alternate Names
      • CXXC-type zinc finger protein 3
      • Methyl-CpG-binding protein MBD1
      • Protein containing methyl-CpG-binding domain 1
      • methyl-CpG binding domain protein 1
      • methyl-CpG binding domain protein 1 isoform PCM1
      • the regulator of fibroblast growth factor 2 (FGF-2)
        transcription
      Background InformationDNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, regulates gene expression and is critical for proper development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that each contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.09% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MBD1 Antibody, C-terminus is a Rabbit Polyclonal Antibody for detection of MBD1 also known as CXXC-type zinc finger protein 3 & has been validated in WB, ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
      Biological Information
      ImmunogenThe immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human MBD1.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes human MBD1.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not tested in other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.

      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

      Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
      Gene Symbol
      • CXXC3
      • OTTHUMP00000163504
      • OTTHUMP00000163506
      • OTTHUMP00000163507
      • PCM1
      • RFT
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION:Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. Ref.1 Ref.3 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.21

      SUBUNIT STRUCTURE: Binds OASL, AFT7IP and AFT7IP2. Binds CHAF1A and the SUV39H1-CBX5 complex via the MBD domain. Binds MGP via the TRD domain. May be part of the MeCP1 complex. During DNA replication, it recruits SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1.

      SUBCELLULAR LOCATION: Nucleus speckle. Note= Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. Ref.1 Ref.3 Ref.8 Ref.9 Ref.12 Ref.21

      TISSUE SPECIFICITY: Widely expressed. Ref.1

      INDUCTION: Up-regulated by interferon. Ref.4

      DOMAIN:The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.

      The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.

      The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

      PTM: Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP. Ref.16 Ref.17

      Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.16 Ref.15 Ref.18 Ref.19

      SEQUENCE SIMILARITY: Contains 3 CXXC-type zinc fingers.

      Contains 1 MBD (methyl-CpG-binding) domain.

      Molecular Weight~61 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      This antibody recognizes MBD1 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications