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ST1622 Anti-LEPRE1 Mouse mAb (3C7)

ST1622
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Key Specifications Table

HostAntibody Type
M Monoclonal Antibody

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ST1622-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.
      Catalogue NumberST1622
      Brand Family Calbiochem®
      SynonymsAnti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1
      Application Data
      Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence.

      Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesChoi, JW, et al. 2009. PLoS Genet. 5, e1000750.
      Cabral, W,A., et al. 2007. Nat Genet. 39, 359.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, Human esophagus tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (6 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
      Clone3C7
      HostMouse
      IsotypeIgG2a
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-LEPRE1 Mouse mAb (3C7) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Choi, JW, et al. 2009. PLoS Genet. 5, e1000750.
      Cabral, W,A., et al. 2007. Nat Genet. 39, 359.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-November-2010 RFH
      SynonymsAnti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (6 µg/ml)
      Application Data
      Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence.

      Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
      BackgroundThis gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
      Clone3C7
      IsotypeIgG2a
      Positive controlHeLa cells, Human esophagus tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesChoi, JW, et al. 2009. PLoS Genet. 5, e1000750.
      Cabral, W,A., et al. 2007. Nat Genet. 39, 359.