407143 Anti-IP₃ Receptor Rabbit pAb

407143
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, Ca, H, M, Porcine, R Rb Polyclonal Antibody

Pricing & Availability

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407143-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes all of the ~260 kDa IP3 receptor subtypes.
      Catalogue Number407143
      Brand Family Calbiochem®
      References
      ReferencesBourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
      Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
      Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunofluorescence
      Immunohistochemistry
      Immunoprecipitation
      Radioimmunoassay
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Application NotesELISA (1:2000)
      Flow Cytometry (1:1000)
      Immunoblotting (1:250)
      Immunofluorescence (1:200)
      Immunohistochemistry (1:200)
      Immunoprecipitation (1:200)
      Radioimmunoassay (1:10000)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.

      Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies

      NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.

      • TBST
      • 20 mM Tris-HCl, pH 7.6
      • 150 mM NaCl
      • 0.05% Tween®-20 detergent

      1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
      2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
      3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
      4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
      5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
      6. Shake for 1 h.
      7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
      8. Mix development solution (1:1) (Amersham ECL kit).
      9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Porcine
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-IP₃ Receptor Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IP₃ Receptor Rabbit pAb Certificates of Analysis

      TitleLot Number
      407143

      References

      Reference overview
      Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
      Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
      Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationELISA (1:2000)
      Flow Cytometry (1:1000)
      Immunoblotting (1:250)
      Immunofluorescence (1:200)
      Immunohistochemistry (1:200)
      Immunoprecipitation (1:200)
      Radioimmunoassay (1:10000)
      DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP3 receptor protein.
      BackgroundInositol 1,4,5-triphosphate (IP3) receptors have been shown to play an important role in the regulation of Ca2+ release from internal storage sites during cell activation.
      HostRabbit
      Immunogena synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
      IsotypeIgG
      Speciesbovine, canine, human, mouse, porcine, rat
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the proper working dilution.

      Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies

      NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.

      • TBST
      • 20 mM Tris-HCl, pH 7.6
      • 150 mM NaCl
      • 0.05% Tween®-20 detergent

      1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
      2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
      3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
      4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
      5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
      6. Shake for 1 h.
      7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
      8. Mix development solution (1:1) (Amersham ECL kit).
      9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
      Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
      Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.