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GR11L Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

GR11L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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GR11L-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
      Catalogue NumberGR11L
      Brand Family Calbiochem®
      SynonymsAnti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor
      References
      ReferencesRoth, R. 1988. Science 239, 1269.
      Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
      Rosen, O.M. 1987. Science 257, 1452.
      Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
      Ullrich, A., et al. 1986. EMBO J. 5, 2503.
      Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
      Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
      Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
      Negative controlHS27 cells
      Positive controlHepG2 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesNeutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
      Key Applications Not Paraffin Sections
      Neutralization Studies
      Immunofluorescence
      Immunoprecipitation
      Not Western Blot
      Application NotesImmunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Neutralization Studies (1 µg/ml; see application references)
      Paraffin Sections (not recommended)
      Immunoblotting (not recommended)
      Application CommentsBlocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human placenta IGF-I receptor
      ImmunogenHuman
      CloneαIR3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) Certificates of Analysis

      TitleLot Number
      GR11L

      References

      Reference overview
      Roth, R. 1988. Science 239, 1269.
      Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
      Rosen, O.M. 1987. Science 257, 1452.
      Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
      Ullrich, A., et al. 1986. EMBO J. 5, 2503.
      Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
      Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
      Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

      Citations

      Title
    • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
    • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
    • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-August-2007 RFH
      SynonymsAnti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor
      ApplicationImmunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Neutralization Studies (1 µg/ml; see application references)
      Paraffin Sections (not recommended)
      Immunoblotting (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
      BackgroundThe insulin-like growth factors (IGFs) or somatomedins are mitogenic peptides present in the plasma of human, rat, and other species. Two distinct but chemically related IGFs have been purified from human plasma: IGF-I (Somatomedin C) and IGF-II. They are single chain polypeptides with molecular weights of 7,640 and 7,471, respectively. Types I and II IGF receptors differ in their structure and in their peptide binding specificity. Type I IGF receptors are structurally homologous to insulin receptors and contain a-subunits (MW = 130,000) that bind peptide, and b-subunits (MW = 90,000), which have intrinsic tyrosine kinase activity. The protein-tyrosine kinase activity of the insulin receptor and, presumably the IGF-I receptor, is required for insulin to stimulate all of its biological responses in cells. Type II receptors are a single polypeptide (MW = 260,000), lack protein-tyrosine kinase activity, and have been shown to be identical to the mannose-6-phosphate receptor.
      HostMouse
      Immunogen speciesHuman
      Immunogenpartially purified, human placenta IGF-I receptor
      CloneαIR3
      IsotypeIgG₁
      Specieshuman
      Positive controlHepG2 cells
      Negative controlHS27 cells
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
      PreservativeNone
      CommentsBlocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesRoth, R. 1988. Science 239, 1269.
      Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
      Rosen, O.M. 1987. Science 257, 1452.
      Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
      Ullrich, A., et al. 1986. EMBO J. 5, 2503.
      Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
      Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
      Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
      Citation
    • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
    • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
    • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
    • Application referencesNeutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

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      Life Science Research > Antibodies and Assays > Primary Antibodies