386040 Anti-Hsp90 Mouse mAb (AC88)

386040
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of Species M Monoclonal Antibody

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
386040-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~90 kDa Hsp90 protein.
      Catalogue Number386040
      Brand Family Calbiochem®
      SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
      Application Data
      Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.
      References
      ReferencesHarry, J.L. et al. 1990. Development 190, 305.
      Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
      Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
      Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 50% glycerol, pH 7.2.
      Positive controlHeat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml, colorimetric)
      Immunoprecipitation (see comments)
      Immunocytochemistry (see comments)
      Application CommentsCross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
      Biological Information
      ImmunogenHsp90 isolated from water mold, Achyla ambisexualis
      ImmunogenAchyla ambisexualis
      CloneAC88
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Hsp90 Mouse mAb (AC88) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Hsp90 Mouse mAb (AC88) Certificates of Analysis

      TitleLot Number
      386040

      References

      Reference overview
      Harry, J.L. et al. 1990. Development 190, 305.
      Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
      Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
      Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
      ApplicationImmunoblotting (1 µg/ml, colorimetric)
      Immunoprecipitation (see comments)
      Immunocytochemistry (see comments)
      Application Data
      Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
      BackgroundHsp90 (heat shock protein 90) is a primarily cytoplasmic protein which exists in dimeric form. Hsp90 binds several cellular proteins such as retrovirus kinases (pp60v-src), steroid receptors, heme-regulated protein kinase, actin, and tubulin. Although Hsp90 expression is induced by stress, and thus may be an indicator of cell stress, its constitutive level may be up to 1-2% of the total cellular protein.
      HostMouse
      Immunogen speciesAchyla ambisexualis
      ImmunogenHsp90 isolated from water mold, Achyla ambisexualis
      CloneAC88
      IsotypeIgG₁
      Speciesa broad range of species
      Positive controlHeat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
      FormLiquid
      FormulationIn 10 mM PBS, 50% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsCross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHarry, J.L. et al. 1990. Development 190, 305.
      Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937.
      Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
      Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.