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ST1529 Anti-HTT Mouse mAb (3F1)

ST1529
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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ST1529-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.
      Catalogue NumberST1529
      Brand Family Calbiochem®
      SynonymsAnti-HD, Anti-Huntingtin
      Application Data
      Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlU-2 OS cells, HeLa NE cells, Human salivary gland tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
      Clone3F1
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-HTT Mouse mAb (3F1) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-HD, Anti-Huntingtin
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa HTT protein.
      BackgroundHuntington's disease is a gene-linked neurodegenerative disorder characterized by loss of striatal neurons. This disorder is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is ~13.7 kb and is expressed predominantly in adult and fetal brain, whereas the smaller transcript is ~10.3 kb and is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
      Clone3F1
      IsotypeIgG2a
      Specieshuman
      Positive controlU-2 OS cells, HeLa NE cells, Human salivary gland tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review