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393207 Anti-HNE-Michael Adducts, Reduced Rabbit pAb

393207
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of Species Rb Polyclonal Antibody

Pricing & Availability

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393207-100UL
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      Plastic ampoule 100 ul
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      Description
      OverviewRecognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.
      Catalogue Number393207
      Brand Family Calbiochem®
      SynonymsAnti-4-Hydroxy-2-noneal Michael Adducts, Reduced
      References
      ReferencesToyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
      Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
      Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
      Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
      Uchida, K., et al. 1994. Biochemistry 33, 12487.
      Product Information
      FormLiquid
      FormulationSerum diluted in 50% glycerol, pH 7.5.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesELISA (1:500-1:1000)
      Immunoblotting (1:1000-1:4000)
      Frozen Sections (1:50-1:500)
      Paraffin Sections (1:50-1:500)
      Application CommentsDoes not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      ImmunogenHNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-HNE-Michael Adducts, Reduced Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-HNE-Michael Adducts, Reduced Rabbit pAb Certificates of Analysis

      TitleLot Number
      393207

      References

      Reference overview
      Toyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
      Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
      Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
      Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
      Uchida, K., et al. 1994. Biochemistry 33, 12487.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      SynonymsAnti-4-Hydroxy-2-noneal Michael Adducts, Reduced
      ApplicationELISA (1:500-1:1000)
      Immunoblotting (1:1000-1:4000)
      Frozen Sections (1:50-1:500)
      Paraffin Sections (1:50-1:500)
      DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.
      BackgroundThe CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer's disease.
      HostRabbit
      ImmunogenHNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
      IsotypeIgG
      Speciesa broad range of species
      FormLiquid
      FormulationSerum diluted in 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsDoes not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesToyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
      Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
      Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
      Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
      Uchida, K., et al. 1994. Biochemistry 33, 12487.