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IF03L Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5)

IF03L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ch, H, Porcine, R M Monoclonal Antibody

Pricing & Availability

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IF03L-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes GFAP in astrocytes, astrocytomas, glial cells, gliomas, and other glial cell-derived tumors.
      Catalogue NumberIF03L
      Brand Family Calbiochem®
      SynonymsAnti-GFAP
      References
      ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
      Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
      Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
      Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
      Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K. 1982. Cell 31, 303.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
      Negative controlEpithelial cells
      Positive controlGlial cells (such as U343/333MG)
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.
      Key Applications Immunoprecipitation
      Paraffin Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (see application references)
      Paraffin Sections (see application references)
      Frozen Sections (see comments)
      Immunofluorescence (see application references)
      Immunoprecipitation (see comments)
      Application CommentsStains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenGFAP
      ImmunogenPorcine
      CloneG-A-5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Chicken
      • Human
      • Porcine
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5) Certificates of Analysis

      TitleLot Number
      IF03L

      References

      Reference overview
      Droese, M., et al. 1984. Acta. Cytol. 28, 368.
      Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
      Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
      Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
      Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K. 1982. Cell 31, 303.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-GFAP
      ApplicationImmunoblotting (see application references)
      Paraffin Sections (see application references)
      Frozen Sections (see comments)
      Immunofluorescence (see application references)
      Immunoprecipitation (see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with PAI mouse myeloma cells (see application references). Recognizes the glial fibrillary acidic protein (GFAP).
      BackgroundIntermediate (10 nm) filaments are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (glial fibrillary acidic protein or GFA or GFAP), skeletal, visceral, and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). GFAP and vimentin are coexpressed in at least some astrocytes. Anti-GFAP (Ab-1) identifies the GFAP subclass of intermediate filaments in human tissues.
      HostMouse
      Immunogen speciesPorcine
      ImmunogenGFAP
      CloneG-A-5
      IsotypeIgG₁
      Specieschicken, human, porcine, rat
      Positive controlGlial cells (such as U343/333MG)
      Negative controlEpithelial cells
      FormLyophilized
      FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
      PreservativeNone
      CommentsStains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
      Toxicity Standard Handling
      ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
      Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
      Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
      Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
      Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K. 1982. Cell 31, 303.
      Application referencesImmunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.