371726 Anti-Giα-3- and Goα-Subunits, C-Terminal (345-354) Rabbit pAb

371726
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ma Rb Polyclonal Antibody

Pricing & Availability

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371726-50UL
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      Glass bottle 50 ul
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      Description
      OverviewRecognizes both Giα-3- and Goα-subunits. Does not cross-react with other G protein subunits.
      Catalogue Number371726
      Brand Family Calbiochem®
      References
      ReferencesRaymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Product Information
      FormLiquid
      FormulationSerum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsThe Giα-3- and Goα-subunits share the last four amino acids (CGLY) of the immunizing peptide. Does not cross-react with other G-proteins. The specificity and cross-reactivity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (KNNLKECGLY) corresponding to amino acids 345-354 of mammalian Giα-3, conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mammals
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Giα-3- and Goα-Subunits, C-Terminal (345-354) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Giα-3- and Goα-Subunits, C-Terminal (345-354) Rabbit pAb Certificates of Analysis

      TitleLot Number
      371726

      References

      Reference overview
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-November-2017 JSW
      ApplicationImmunoblotting (1:1000)
      DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes Giα-3 and Goα subunit proteins.
      HostRabbit
      Immunogena synthetic peptide (KNNLKECGLY) corresponding to amino acids 345-354 of mammalian Giα-3, conjugated to KLH
      IsotypeIgG
      Speciesmammalian
      FormLiquid
      FormulationSerum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      CommentsThe Giα-3- and Goα-subunits share the last four amino acids (CGLY) of the immunizing peptide. Does not cross-react with other G-proteins. The specificity and cross-reactivity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesRaymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.