ST1543 Anti-GHR Mouse mAb (3A12)

ST1543
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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ST1543-100UG
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      100 μg
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      Description
      OverviewRecognizes the GHR protein in HeLa cells.
      Catalogue NumberST1543
      Brand Family Calbiochem®
      SynonymsAnti-Growth Hormone Receptor
      Application Data
      Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (1:50)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
      Clone3A12
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-GHR Mouse mAb (3A12) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-Growth Hormone Receptor
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (1:50)
      Application Data
      Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
      DescriptionPurified mouse monoclonal antibody. Recognizes the GHR protein.
      BackgroundGHR is a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
      Clone3A12
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review