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GR13 Anti-EGFR (Ab-3) Mouse mAb (225)

GR13
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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GR13-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~170 kDa epidermal growth factor receptor protein. For neutralization studies Cat. No. GR13L is recommended.
      Catalogue NumberGR13
      Brand Family Calbiochem®
      SynonymsAnti- Epidermal Growth Factor Receptor
      References
      ReferencesHunter T. 1984. Nature 311, 414.
      Reynolds, F.H., et. al 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlHL-60 cells
      Positive controlA-431 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.
      Key Applications Immunoprecipitation
      Application NotesImmunoprecipitation (1-2 µg per sample)
      Neutralization Studies (2 µg/ml, see application references; use Cat. No. GR13L)
      Application CommentsShown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. For neutralization studies GR13L is recommended. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human EGFR from A431 cells
      ImmunogenHuman
      Clone225
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-EGFR (Ab-3) Mouse mAb (225) Certificates of Analysis

      TitleLot Number
      GR13

      References

      Reference overview
      Hunter T. 1984. Nature 311, 414.
      Reynolds, F.H., et. al 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 JSW
      SynonymsAnti- Epidermal Growth Factor Receptor
      ApplicationImmunoprecipitation (1-2 µg per sample)
      Neutralization Studies (2 µg/ml, see application references; use Cat. No. GR13L)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
      BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF receptor is phosphorylated on tyrosine residues. Subsequent to binding, EGF receptor complexes are internalized and cell proliferation takes place with partially-purified EGF receptor from human A431 epidermal carcinoma cells.
      HostMouse
      Immunogen speciesHuman
      Immunogenpartially purified, human EGFR from A431 cells
      Clone225
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlA-431 cells
      Negative controlHL-60 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsShown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. For neutralization studies GR13L is recommended. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesHunter T. 1984. Nature 311, 414.
      Reynolds, F.H., et. al 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Application referencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies