GR01L Sigma-AldrichAnti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Anti-EGFR (Ab-1) Mouse mAb (528) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Synonyms: Anti-Epidermal Growth Factor Receptor

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Overview

Replacement Information

Key Specifications Table

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

Pricing & Availability

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Description
Overview	Recognizes the EGF receptor protein in A431 cells and normal skin.
Catalogue Number	GR01L
Brand Family	Calbiochem®
Synonyms	Anti-Epidermal Growth Factor Receptor

References
References	Hunter T. 1984. Nature 311, 414. Masui, H., et al. 1984. Cancer Res. 44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

Product Information
Form	Lyophilized
Formulation	Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
Positive control	A-431 cells or skin tissue
Preservative	None
Quality Level	MQ100

Applications
Application References	Original Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.
Key Applications	Immunoprecipitation Neutralization Studies Paraffin Sections Flow Cytometry Frozen Sections Immunofluorescence
Application Notes	Flow Cytometry (5 µg/ml) Frozen Sections (2.5 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (see comments and application references) Paraffin Sections (see application references)
Application Comments	This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	partially purified, human EGFR from human A431 cells
Immunogen	Human
Clone	528
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

Packaging Information

Transport Information

Supplemental Information

Specifications

Documentation

Anti-EGFR (Ab-1) Mouse mAb (528) SDS

Title
Safety Data Sheet (SDS)

Anti-EGFR (Ab-1) Mouse mAb (528) Certificates of Analysis

Title	Lot Number
GR01L	Enter Lot Number

References

Reference overview
Hunter T. 1984. Nature 311, 414. Masui, H., et al. 1984. Cancer Res. 44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	25-October-2007 JSW
Synonyms	Anti-Epidermal Growth Factor Receptor
Application	Flow Cytometry (5 µg/ml) Frozen Sections (2.5 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (see comments and application references) Paraffin Sections (see application references)
Description	Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
Background	Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
Host	Mouse
Immunogen species	Human
Immunogen	partially purified, human EGFR from human A431 cells
Clone	528
Isotype	IgG_2a
Species	human, not mouse, not rat
Positive control	A-431 cells or skin tissue
Form	Lyophilized
Formulation	Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
Preservative	None
Comments	This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
Toxicity	Standard Handling
References	Hunter T. 1984. Nature 311, 414. Masui, H., et al. 1984. Cancer Res. 44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Application references	Original Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.

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