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ABC1389 Anti-DCLK1-S

ABC1389
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1389
      DescriptionAnti-DCLK1-S
      Alternate Names
      • Serine/threonine-protein kinase DCLK1-S
      • EC: 2.7.11.1
      • Doublecortin domain-containing protein 3A-short
      • Doublecortin-like and CAM kinase-like 1
      • Doublecortin-like kinase 1
      • PS41014
      Background InformationSerine/threonine-protein kinase DCLK1 (UniProt: O15075; also known as EC: 2.7.11.1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1) is encoded by the DCLK1 (also known as DCAMKL1, DCDC3A, KIAA0369) gene (Gene ID: 9201) in human. DCLK1 is an integral component of plasma membrane and is highly expressed in fetal brain tissue and is also detectable in lung and liver. DCLK1 expression is reported to be critical for growth of human colon cancer cells and its expression is up-regulated in response to growth factors resulting in hyperproliferation. Both long (L) (~80-82 kDa) and short (S) (~45-50 kDa) isoforms of DCLK1 have been described. The long isoform of DCLK1 contains two N-terminal doublecortin domains, which bind microtubules, a C-terminal serine/threonine kinase domain with homology to CaM kinases, and a middle serine/proline rich domain that mediates protein interactions. The short isoform, DCLK1-S, has been reported in human colorectal cancer cells and in cancer stem cells, while normal colon cells express only the long isoform of DCLK1. The short isoforms lacks the two N-terminal doublecortin domains. Although DCLK1-S differs from the long isoform by only six amino-acids., this mono-specific antibody does not cross-react with the long isoform and is shown to specifically detect cancer stem cells. The short isoform is not a splice variant, but is expressed from an alternate beta promoter in intron V of the gene that results in the presence of the unique sequence of amino acids. These amino acids are absent in the long isoform, which is expressed by the alpha promoter in the 5’ region of the gene. It has been reported that the 5' (alpha)-promoter of the human DCLK1 gene becomes epigenetically silenced during colon carcinogenesis, resulting in loss of expression of the canonical long isoform in human colorectal adenocarcinomas (hCRCs). Instead, hCRCs express the short isoform, DCLK1-S. Colon tumors and normal colons from mice only express the long isoform, since the epigenetics of colon carcinogenesis in mice is significantly different from that in humans. (Ref.: Sarkar, S et al. (2017). Lab. Invest. doi:10. 1038/labinvest.2017.40; Sarkar, S., et al. (2017). Mol. Cancer Res. doi: 10.1158/1541-7786.MCR-17-0287).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-DCLK1-S, Cat. No. ABC1389, is a rabbit polyclonal antibody that specifically detects the short version of Serine/threonine-protein kinase DCLK1. It has been tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected DCLK1-S in Immunohistochemistry applications (Sarkar, S., et. al. (2017). Lab Invest. [Epub ahead of print]).

      Western Blotting Analysis: A representative lot detected DCLK1-S in Western Blotting applications (Sarkar, S., et. al. (2017). Lab Invest. [Epub ahead of print]).
      Biological Information
      ImmunogenA multimeric scaffold of the 6 amino acids from the N-terminal region of the short version of human DCLK1 (DCLK1-S).
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects the short version of Serine/threonine-protein kinase DCLK1 (DCLK1-S). It does not react with long isoforms. It targets an epitope within six amino acids from the N-terminus region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DCLK1
      • DCAMKL1
      • ;DCDC3A
      • KIAA0369
      • DCLK1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~55 kDa observed. 47.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected DCLK1-S in 10 µg of HCT116 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications