69050 Anti-Cre Antibody

69050
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69050-3
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      Plastic ampoule 50 ul
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      Description
      OverviewRecombinase cre (UniProt: P06956; also known as Cre) is encoded by the cre gene (Gene ID: 2777477) in Escherichia phage P1 (Bacteriophage P1). Cre (Causes recombination with Cyclization recombinase), a 38 kDa DNA recombinase of the 'phage' integrase family, is derived from the P1 bacteriophage. It catalyzes site-specific recombination and is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each bind to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals in vivo.
      Supplied as purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide and 50% glycerol.

      Evaluated by Western Blotting with Recombinant Cre-His.

      Western Blotting Analysis: Representative lot data. Recombinant Cre-His loaded at 20 ng/lane (Lane 1) and 10 ng/lane (Lane 2) was probed with Cat. No. 69050-3, Anti-Cre (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cre (~39 kDa).
      Catalogue Number69050
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
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      Safety Information according to GHS
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      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Supplemental Information
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      Documentation

      Anti-Cre Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cre Antibody Certificates of Analysis

      TitleLot Number
      69050

      Citations

      Title
    • Qian, J.Y., et al. 2008. Hypertension 51, 560.
    • Dworniczak, B., et al. 2007. Nephron Experimental Nephrology 106, e11.
    • Froment, P., et al. 2007. Journal of Endocrinology 194, 557.
    • Tiedt, R., et al. 2007. Blood 109, 1503.
    • Davé, V., et al. 2006. Journal of Clinical Investigation 116, 2597.
    • Forni, P.E., et al. 2006. Journal of Neuroscience 26, 9593.
    • Gao, F., et al. 2006. Procedings of the National Academy of Science 103, 11987.
    • Masahira, N., et al. 2006. Developmental Biology 293, 358.
    • Rajji, T., et al. 2006. Journal of Neuroscience 26, 908.
    • Wei, Q., et al. 2006. Cancer Research 66, 7429.
    • Xie, y., et al. 2006. Journal of Biological Chemistry 281, 4075.
    • Balthasar N., et al. 2005. Cell 123, 493.
    • Izumi, T., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 5739.
    • Koike, C., et al. 2005. Journal of Neuroscience 25, 10290.
    • Moeller, M.J., et al. 2005. American Journal of Physiology: Renal 289, F481.
    • Pfister, O., et al. 2005. Circulation Research 97, 52.
    • Schulein, R., et al. 2005. Procedings of the National Academy of Science 102, 856.
    • Peitz, M., et al. 2002. Procedings of the National Academy of Science 99, 4489.
    • Takebayashi, H., et al. 2002. Current Biology 12, 1157.
    • Morimura, T., et al. 2000. Journal of Biological Chemistry 275, 36523.
    • Selbert, S., et al. 1998. Transgenic Research 7, 387.
    • Giovannini, et. al. 2000. Genes and Development 14, 1617.
    • User Protocols

      Title
      TB421 Anti-Cre Antibody