AB5966 Anti-Choline Transporter Antibody, high affinity

AB5966
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5966
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Transporter Antibody, high affinity
      Alternate Names
      • CHT
      • HAChT
      References
      Product Information
      FormatPurified
      PresentationLiquid.
      Applications
      ApplicationAnti-Choline Transporter Antibody, high affinity detects level of Choline Transporter & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:1,000 The antibody reacts with a ~70 kDa protein in mouse spinal cord and a doublet of ~70-75 kDa in mouse striatum tissue homogenate samples.

      Immunohistochemistry: 1:100-1:500 in mouse heart.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogenfull length recombinant human CHT fusion protein.
      Epitopehigh affinity
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityCholine Transporter, High Affinity (CHT).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCholine is a direct precursor of acetylcholine (ACh), a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic, cognitive, and motor functions. SLC5A7 is a Na(+)- and Cl(-)- dependent high-affinity transporter that mediates the uptake of choline for acetylcholine synthesis in cholinergic neurons (Apparsundaram et al., 2000 [PubMed 11027560]).
      Gene Symbol
      • SLC5A7
      • CHT
      • CHT1
      • hCHT
      • MGC126299
      • MGC126300
      UniProt Number
      UniProt SummaryFUNCTION: Imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.
      SIZE: 580 amino acids; 63,204 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in putamen, spinal cord and medulla. Specific for cholinergic neurons.
      SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Choline Transporter Antibody, high affinity SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Choline Transporter Antibody, high affinity Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-CHOLINE TRANSPORTER, HIGH AFFINITY - 21019962101996
      RABBIT ANTI-CHOLINE TRANSPORTER, HIGH AFFINITY POLYCLONAL ANTIBODY - 21507272150727
      RABBIT ANTI-CHOLINE TRANSPORTER, HIGH AFFINITY POLYCLONAL ANTIBODY - 20217332021733
      RABBIT ANTI-CHOLINE TRANSPORTER, HIGH AFFINITY POLYCLONAL ANTIBODY - 20371942037194
      RABBIT ANTI-CHOLINE TRANSPORTER, HIGH AFFINITY POLYCLONAL ANTIBODY - 20609702060970
      RABBIT ANTI-CHOLINE TRANSPORTER, HIGH AFFINITY POLYCLONAL ANTIBODY - 20909272090927

      References

      Reference overviewApplicationSpeciesPub Med ID
      B6eGFPChAT mice overexpressing the vesicular acetylcholine transporter exhibit spontaneous hypoactivity and enhanced exploration in novel environments.
      Nagy, PM; Aubert, I
      Brain and behavior  3  367-83  2013

      Show Abstract
      Western BlottingMouse24381809 24381809
      Functional neuromuscular junctions formed by embryonic stem cell-derived motor neurons.
      Umbach, JA; Adams, KL; Gundersen, CB; Novitch, BG
      PloS one  7  e36049  2012

      Show Abstract
      22574134 22574134
      Effect of choline-containing phospholipids on brain cholinergic transporters in the rat.
      Tayebati SK, Tomassoni D, Di Stefano A, Sozio P, Cerasa LS, Amenta F.
      Journal of the neurological sciences  302  49-57  2011

      Show Abstract
      21195433 21195433
      Alpha cells secrete acetylcholine as a non-neuronal paracrine signal priming beta cell function in humans.
      Rodriguez-Diaz, R; Dando, R; Jacques-Silva, MC; Fachado, A; Molina, J; Abdulreda, MH; Ricordi, C; Roper, SD; Berggren, PO; Caicedo, A
      Nature medicine  17  888-92  2011

      Show Abstract
      Immunohistochemistry21685896 21685896
      Nuclear choline acetyltransferase activates transcription of a high-affinity choline transporter.
      Matsuo, A; Bellier, JP; Nishimura, M; Yasuhara, O; Saito, N; Kimura, H
      The Journal of biological chemistry  286  5836-45  2011

      Show Abstract
      21163949 21163949
      Narcoleptic orexin receptor knockout mice express enhanced cholinergic properties in laterodorsal tegmental neurons.
      Kalogiannis, M; Grupke, SL; Potter, PE; Edwards, JG; Chemelli, RM; Kisanuki, YY; Yanagisawa, M; Leonard, CS
      The European journal of neuroscience  32  130-42  2010

      Show Abstract Full Text Article
      Western Blotting20576035 20576035
      Cholinergic neurons of mouse intrinsic cardiac ganglia contain noradrenergic enzymes, norepinephrine transporters, and the neurotrophin receptors tropomyosin-related kinase A and p75.
      Hoard, JL; Hoover, DB; Mabe, AM; Blakely, RD; Feng, N; Paolocci, N
      Neuroscience  156  129-42  2008

      Show Abstract Full Text Article
      ImmunohistochemistryMouse18674600 18674600
      High affinity choline transporter immunoreactivity in rat ileum myenteric nerves.
      Andrea M Harrington, J M Hutson, Bridget R Southwell
      Cell and tissue research  327  421-31  2007

      Show Abstract
      17093920 17093920
      Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture.
      Hoard, JL; Hoover, DB; Wondergem, R
      American journal of physiology. Cell physiology  293  C1875-83  2007

      Show Abstract
      ImmunohistochemistryMouse17913847 17913847
      Prenatal choline deficiency increases choline transporter expression in the septum and hippocampus during postnatal development and in adulthood in rats.
      Mellott, TJ; Kowall, NW; Lopez-Coviella, I; Blusztajn, JK
      Brain research  1151  1-11  2007

      Show Abstract
      17399691 17399691

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies