AB1582 Anti-Choline Acetyltransferase Antibody

AB1582
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, R, Rb IHC, WB Sh Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1582
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase Antibody
      Alternate Names
      • ChAT
      References
      Product Information
      FormatSerum
      PresentationSheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry: 1:1,000-1:2,000

      Immunoblotting

      Optimal working dilutions must be determined by end user.

      IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582

      STAINING OF GUINEA PIG ILEUM

      1) Pin the ileum out flat and tight.

      2) Fix overnight at 4°C in modified Zamboni's (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).

      3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.

      4) Dissect off ALL circular muscle.

      5) Preincubate with 10% normal donkey serum.

      6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.

      7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.

      IMPORTANT NOTE: It is essential to remove ALL circular muscle.
      Biological Information
      ImmunogenA 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2.
      HostSheep
      SpecificityCholine Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems.
      Species Reactivity
      • Guinea Pig
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
      Gene Symbol
      • CHAT
      • ChAT
      • CMS1A2
      • CHOACTase
      • CMS1A
      • EC 2.3.1.6
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Choline Acetyltransferase Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Choline Acetyltransferase Antibody Certificates of Analysis

      TitleLot Number
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT)2470377
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 21159122115912
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 21224302122430
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 23839522383952
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) - 22193272219327
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) - 33290623329062
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) -27897252789725
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY2908692
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) POLYCLONAL ANTIBODY - 23220302322030

      References

      Reference overviewPub Med ID
      Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation.
      Jiang, YH; Jiang, P; Yang, JL; Ma, DF; Lin, HQ; Su, WG; Wang, Z; Li, X
      PloS one  10  e0133971  2015

      Show Abstract
      26230083 26230083
      Immunohistochemical localization of calbindin-D28k and calretinin in the brainstem of anuran and urodele amphibians.
      Ruth Morona, Agustin González, Ruth Morona, Agustin González, Ruth Morona, Agustin González
      The Journal of comparative neurology  515  503-37  2009

      Show Abstract
      19479990 19479990
      Vesicular glutamate transporter 1 and vesicular glutamate transporter 2 synapses on cholinergic neurons in the sublenticular gray of the rat basal forebrain: a double-label electron microscopic study.
      Hur, EE; Edwards, RH; Rommer, E; Zaborszky, L
      Neuroscience  164  1721-31  2009

      Show Abstract
      19778580 19778580
      Neural pathways underlying lactate-induced panic.
      Johnson, PL; Truitt, WA; Fitz, SD; Lowry, CA; Shekhar, A
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  33  2093-107  2008

      Show Abstract
      18059441 18059441
      Immuno-localization of vesicular acetylcholine transporter in mouse taste cells and adjacent nerve fibers: indication of acetylcholine release.
      Tatsuya Ogura,Robert F Margolskee,Yvonne N Tallini,Bo Shui,Michael I Kotlikoff,Weihong Lin
      Cell and tissue research  330  2007

      Show Abstract
      17704949 17704949
      Synaptic density, convergence, and dendritic complexity of prevertebral sympathetic neurons.
      Ian L Gibbins, Ee Hiok Teo, Phillip Jobling, Judy L Morris
      The Journal of comparative neurology  455  285-98  2003

      Show Abstract
      12483682 12483682
      Heterogeneous expression of SNAP-25 and synaptic vesicle proteins by central and peripheral inputs to sympathetic neurons.
      Ian L Gibbins, Phillip Jobling, Ee Hiok Teo, Sue E Matthew, Judy L Morris, Ian L Gibbins, Phillip Jobling, Ee Hiok Teo, Sue E Matthew, Judy L Morris
      The Journal of comparative neurology  459  25-43  2003

      Show Abstract
      12629665 12629665
      Self-secretion of fibroblast growth factor-9 supports basal forebrain cholinergic neurons in an autocrine/paracrine manner.
      T Kanda, T Iwasaki, S Nakamura, T Kurokawa, K Ikeda, H Mizusawa, T Kanda, T Iwasaki, S Nakamura, T Kurokawa, K Ikeda, H Mizusawa
      Brain research  876  22-30  2000

      Show Abstract
      10973589 10973589
      Muscular innervation of the proximal duodenum of the guinea pig.
      S Iino
      Archives of histology and cytology  63  327-43  2000

      Show Abstract
      11073065 11073065
      Subpopulations of sympathetic neurons project to specific vascular targets in the pinna of the rabbit ear.
      J L Morris, B S Zhu, I L Gibbins, W W Blessing
      The Journal of comparative neurology  412  147-60  1999

      Show Abstract
      10440716 10440716

      Data Sheet

      Title
      SHEEP ANTI-CHOLINE ACETYLTRANSFERASE (ChAT)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies