AB144P Anti-Choline Acetyltransferase Antibody

AB144P
500 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Mk, Op, Av, Ch, Gp, Zebrafish IH(P), ICC, IHC, WB Gt Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB144P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase Antibody
      OverviewCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      Alternate Names
      • Choline acetylase
      • acetyl CoA:choline O-acetyltransferase
      • choline acetyltransferase
      • ChAT
      Background InformationAcetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human placenta lysates, rat forebrain/rostral hypothalamus.
      PresentationPurified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Choline Acetyltransferase Antibody detects level of ChAT and has been published and validated for use in IH(P), IC, IH and WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was used at 1:100.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenHuman placental enzyme.
      HostGoat
      SpecificityCholine acetyltransferase found in cholinergic neurons in brain and spinal cord.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      • Opossum
      • Avian
      • Chicken
      • Guinea Pig
      • Zebrafish
      Species Reactivity NoteHuman and mouse. Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
      Demonstrated to react in zebrafish in THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
      Gene Symbol
      • CHAT
      • ChAT
      • CHOACTase
      • CMS1A
      • CMS1A2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Molecular Weight70/74 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
      For short term storage (3-4 weeks) maintain at 2-8°C.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Choline Acetyltransferase Antibody Certificates of Analysis

      TitleLot Number
      Anti-Choline Acetyltransferase2464504
      Anti-Choline Acetyltransferase - 21470412147041
      Anti-Choline Acetyltransferase - 23839492383949
      Anti-Choline Acetyltransferase - 23873962387396
      Anti-Choline Acetyltransferase - 24536442453644
      Anti-Choline Acetyltransferase - 19787471978747
      Anti-Choline Acetyltransferase - 20008012000801
      Anti-Choline Acetyltransferase - 20100602010060
      Anti-Choline Acetyltransferase - 20411072041107
      Anti-Choline Acetyltransferase - 20703922070392

      References

      Reference overviewApplicationSpeciesPub Med ID
      Corelease of acetylcholine and GABA from cholinergic forebrain neurons.
      Saunders, A; Granger, AJ; Sabatini, BL
      eLife  4  2015

      Show Abstract
      25723967 25723967
      Prototypic and arkypallidal neurons in the dopamine-intact external globus pallidus.
      Abdi, A; Mallet, N; Mohamed, FY; Sharott, A; Dodson, PD; Nakamura, KC; Suri, S; Avery, SV; Larvin, JT; Garas, FN; Garas, SN; Vinciati, F; Morin, S; Bezard, E; Baufreton, J; Magill, PJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  6667-88  2015

      Show Abstract
      25926446 25926446
      OTX2 Transcription Factor Controls Regional Patterning within the Medial Ganglionic Eminence and Regional Identity of the Septum.
      Hoch, RV; Lindtner, S; Price, JD; Rubenstein, JL
      Cell reports  12  482-94  2015

      Show Abstract
      26166575 26166575
      Restricted loss of olivocochlear but not vestibular efferent neurons in the senescent gerbil (Meriones unguiculatus).
      Radtke-Schuller, S; Seeler, S; Grothe, B
      Frontiers in aging neuroscience  7  4  2015

      Show Abstract
      25762929 25762929
      Neurite Mistargeting and Inverse Order of Intraretinal Vascular Plexus Formation Precede Subretinal Vascularization in Vldlr Mutant Mice.
      Johnson, V; Xiang, M; Chen, Z; Junge, HJ
      PloS one  10  e0132013  2015

      Show Abstract
      26177550 26177550
      Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool.
      van Wyk, M; Pielecka-Fortuna, J; Löwel, S; Kleinlogel, S
      PLoS biology  13  e1002143  2015

      Show Abstract
      Immunoblotting (Western)25950461 25950461
      Tfap2a and 2b act downstream of Ptf1a to promote amacrine cell differentiation during retinogenesis.
      Jin, K; Jiang, H; Xiao, D; Zou, M; Zhu, J; Xiang, M
      Molecular brain  8  28  2015

      Show Abstract
      25966682 25966682
      Active mechanistic target of rapamycin plays an ancillary rather than essential role in zebrafish CNS axon regeneration.
      Diekmann, H; Kalbhen, P; Fischer, D
      Frontiers in cellular neuroscience  9  251  2015

      Show Abstract
      26217179 26217179
      Hippocampal "cholinergic interneurons" visualized with the choline acetyltransferase promoter: anatomical distribution, intrinsic membrane properties, neurochemical characteristics, and capacity for cholinergic modulation.
      Yi, F; Catudio-Garrett, E; Gábriel, R; Wilhelm, M; Erdelyi, F; Szabo, G; Deisseroth, K; Lawrence, J
      Frontiers in synaptic neuroscience  7  4  2015

      Show Abstract
      25798106 25798106
      Recording, labeling, and transfection of single neurons in deep brain structures.
      Dempsey, B; Turner, AJ; Le, S; Sun, QJ; Bou Farah, L; Allen, AM; Goodchild, AK; McMullan, S
      Physiological reports  3  2015

      Show Abstract
      25602013 25602013

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies