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AB16969 Anti-Caspase 9 Antibody, proform & lg. subunit

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100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbPurifiedPolyclonal Antibody
      Catalogue NumberAB16969
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 9 Antibody, proform & lg. subunit
      Alternate Names
      • ICE-LAP6
      • Mch-6
      • Apaf-3
      Product Information
      HS Code3002 15 90
      PresentationImmunoaffinity chromatography purified immunoglobulin. Liquid in PBS, containing 0.02% sodium azide.
      Quality LevelMQ100
      ApplicationDetect Caspase 9 using this Anti-Caspase 9 Antibody, proform & lg. subunit validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:1,000 to 1:2,000

      Whole cell lysate from HeLa cells can be used as a positive control and a 46 kDa band should be detected.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 41-56 of human caspase-9 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Recognizes proform (46 kDa); sequence analysis predicts cross-reactivity with mouse caspase-9 (>90%).
      Epitopeproform & lg. subunit
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (Nijhawan et al. 1997). Caspase-9 is also activated by granzyme B and CPP32 (Duan et al. 1996; Srinivasula et al. 1996). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Nijhawan et al. 1997). Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis inducers including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD (Pan et al. 1998). Caspase-9 is expressed in a variety of human tissues (Duan et al. 1996; Srinivasula et al. 1996).
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
      Gene Symbol
      • CASP9
      • CASP-9
      • ICE-LAP6
      • APAF3
      • MCH6
      • CASPASE-9c
      • OTTHUMP00000044594
      • APAF-3
      • EC [Contains: Caspase-9 subunit p35
      • Caspase-9 subunit p10].
      Non-Reactive Species
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
      SIZE: 416 amino acids; 46281 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.
      TISSUE SPECIFICITY: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues.
      PTM: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      SIMILARITY: SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe undiluted antibody solution is stable for approximately 6 months when stored 2-8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      AB16969 04053252737534


      Anti-Caspase 9 Antibody, proform & lg. subunit SDS


      Safety Data Sheet (SDS) 

      Anti-Caspase 9 Antibody, proform & lg. subunit Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-CASPASE-9 [ICE-LAP6, MCH6, APAF-3] - 3291358 3291358


      Reference overviewPub Med ID
      Mitochondrial-nuclear communication by prohibitin shuttling under oxidative stress.
      Srinivas R Sripathi,Weilue He,Cameron L Atkinson,Joseph J Smith,Zhicong Liu,Beth M Elledge,Wan Jin Jahng
      Biochemistry  50  2011

      Show Abstract
      21879722 21879722
      Lack of pathology in a triple transgenic mouse model of Alzheimer's disease after overexpression of the anti-apoptotic protein Bcl-2.
      Rohn, TT; Vyas, V; Hernandez-Estrada, T; Nichol, KE; Christie, LA; Head, E
      The Journal of neuroscience : the official journal of the Society for Neuroscience  28  3051-9  2008

      Show Abstract
      18354008 18354008
      Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway.
      Srinivasula, S M, et al.
      J. Biol. Chem., 275: 36152-7 (2000)  2000

      Show Abstract
      10950947 10950947
      The receptor for the cytotoxic ligand TRAIL.
      Pan, G, et al.
      Science, 276: 111-3 (1997)  1997

      Show Abstract
      9082980 9082980

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      Life Science Research > Antibodies and Assays > Primary Antibodies