Millipore Sigma Vibrant Logo

MAB4708 Anti-Caspase 8 Antibody, clone 1-1-37

View Products on Sigmaaldrich.com
MAB4708
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4708
      Replaces04-573; 04-574
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 8 Antibody, clone 1-1-37
      Alternate Names
      • MACH1
      • FLICE
      Background InformationCaspase-8 is a 55 kDa protein that binds to the death effector domain (DED) of FADD through an analogous DED domain present in the proform of caspase-8. Recruitment of caspase-8 to the Fas receptor results in oligomerization of the caspase-8 protein, which drives its auto-activation through self-cleavage. Active caspase-8 then proteolytically activates other downstream caspases including caspase-9, which lead to cell death.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Caspase 8 using this Anti-Caspase 8 Antibody, clone 1-1-37 validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:100 - 1:1000

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant full-length human caspase-8 protein
      Clone1-1-37
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHighly specific to caspase-8 and shows no cross-reaction with other caspase family members. This antibody recognizes the full length 55kDa caspase 8 on western blots.
      IsotypeIg
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
      Gene Symbol
      • CASP8
      • MCH5
      • MGC78473
      • CASP-8
      • MACH
      • ALPS2B
      • FLICE
      • procaspase-8
      • CAP4
      • EC 3.4.22.61 [Contains: Caspase-8 subunit p18
      • Caspase-8 subunit p10].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14790 # Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoforms 5, 6, 7 and 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
      SIZE: 479 amino acids; 55391 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Isoforms 1, 5 and 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus, and liver. Barely detectable in brain, testis, and skeletal muscle.DOMAIN:SwissProt: Q14790 Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
      PTM: Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. & Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE: SwissProt: Q14790 # Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
      SIMILARITY: Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB4708 04053252667817

      Documentation

      Anti-Caspase 8 Antibody, clone 1-1-37 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase 8 Antibody, clone 1-1-37 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CASPASE-8 [FLICE] - 3195929 3195929
      MOUSE ANTI-CASPASE-8 [FLICE] - 3283659 3283659
      MOUSE ANTI-CASPASE-8 [FLICE] - 3718340 3718340
      MOUSE ANTI-CASPASE-8 [FLICE] - 3955341 3955341
      MOUSE ANTI-CASPASE-8 [FLICE] - 4030001 4030001
      MOUSE ANTI-CASPASE-8 [FLICE] - 4037464 4037464
      MOUSE ANTI-CASPASE-8 [FLICE] MONOCLONAL ANTIBODY - 2272690 2272690
      MOUSE ANTI-CASPASE-8 [FLICE] MONOCLONAL ANTIBODY -2601752 2601752
      MOUSE ANTI-CASPASE-8 [FLICE] MONOCLONAL ANTIBODY -2624988 2624988
      MOUSE ANTI-CASPASE-8 [FLICE] MONOCLONAL ANTIBODY -2633781 2633781

      References

      Reference overviewPub Med ID
      Activated Cdc42-associated kinase 1 (Ack1) is required for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor recruitment to lipid rafts and induction of cell death.
      Linderoth, E; Pilia, G; Mahajan, NP; Ferby, I
      The Journal of biological chemistry  288  32922-31  2013

      Show Abstract
      24085293 24085293

      Related Products & Applications

      Related Products

      Catalog Number Description  
      MAB10754 Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies