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06-735 Anti-Caspase 3 Antibody

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHCRbPurifiedPolyclonal Antibody
      Catalogue Number06-735
      Replaces04-1090; 04-439
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Caspase 3 Antibody
      Background InformationCaspase-3 (UniProt: P42574; also known as EC:, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1) is encoded by the CASP3 (also known as CPP32) gene (Gene ID: 836) in human. Cysteine-aspartic proteases or Caspases play essential roles in apoptosis, necrosis, and inflammation. Historically, caspases were numbered in the order in which they were identified. Caspase-3 is a heterotetrameric enzyme that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Caspase-3 is initially produced with a propeptide sequence (aa 1-9), the removal of which yields the 268 aa. caspase-3 proenzyme. Upon activation, the proenzyme is proteolytically cleaved first between Asp175-Ser176 to generate a p20 (aa 10-175) fragment and the p12 (aa 176-277) subunit. Further cleavage of the p20 fragment between Asp28-Ser29 produces the p17 (aa 29-175) subunit. The p17 and p12 subunits dimerize and forms the active caspase-3 enzyme. Caspase-3 has a strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. Caspase-3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a Asp216-|-Gly217 bond. Caspase-3 mediates the proteolytic activation of caspases-6 and -7, while caspase-3 itself is processed and activated by caspase-8, -9, or -10.
      Product Information
      HS Code3002 15 90
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
      PresentationProtein A purified IgG in of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl,and 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationThis Anti-Caspase 3 Antibody is validated for use in Immunohistochemistry and Western Blotting for the detection of Caspase 3.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 1mg/mL of this antibody detects Caspase-3 in A431 cell lysate.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Caspase-3 in Human tonsil tissue sections.
      Biological Information
      ImmunogenHuman full-length Caspase 3 fusion protein containing a histidine-6 tag
      SpecificityRecognizes full-length Caspase 3 (Yama/Apopain) and proteolytic fragments.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
      Gene Symbol
      • CASP3
      • CPP-32
      • Apopain
      • Yama
      • CPP32B
      • apopain
      • procaspase3
      • CASP-3
      • SCA-1
      • CPP32
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
      SIZE: 277 amino acids; 31608 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
      TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
      PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
      SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
      Molecular Weight32 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from non-stimulated human A431 cells, mouse 3T3/A31 or rat PC12 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      06-735 04053252280269


      Anti-Caspase 3 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Caspase 3 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Caspase 3 (rabbit polyclonal IgG) - 2391106 2391106
      Anti-Caspase 3 (rabbit polyclonal IgG) 3055589
      Anti-Caspase 3 (rabbit polyclonal IgG) - 1963624 1963624
      Anti-Caspase 3 (rabbit polyclonal IgG) - 1963624A 1963624A
      Anti-Caspase 3 (rabbit polyclonal IgG) - 2038661 2038661
      Anti-Caspase 3 (rabbit polyclonal IgG) - 2251936 2251936
      Anti-Caspase 3 - 16820 16820
      Anti-Caspase 3 - 18079 18079
      Anti-Caspase 3 - 19227 19227
      Anti-Caspase 3 - 1971286 1971286


      Reference overviewApplicationPub Med ID
      Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells.
      Gastaldello, S; Chen, X; Callegari, S; Masucci, MG
      PLoS pathogens  9  e1003664  2013

      Show Abstract
      24130483 24130483
      Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue.
      Chen, CC; Lin, WC; Kong, MS; Shi, HN; Walker, WA; Lin, CY; Huang, CT; Lin, YC; Jung, SM; Lin, TY
      The British journal of nutrition  107  1623-34  2012

      Show Abstract
      21992995 21992995
      Hepatocyte growth factor is a mouse fetal Leydig cell terminal differentiation factor.
      Ricci, G; Guglielmo, MC; Caruso, M; Ferranti, F; Canipari, R; Galdieri, M; Catizone, A
      Biology of reproduction  87  146  2012

      Show Abstract
      23077169 23077169
      A caspase cascade regulating developmental axon degeneration.
      Simon, DJ; Weimer, RM; McLaughlin, T; Kallop, D; Stanger, K; Yang, J; O'Leary, DD; Hannoush, RN; Tessier-Lavigne, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  17540-53  2012

      Show Abstract
      23223278 23223278
      Differential roles for caspase-mediated and calpain-mediated cell death in 1- and 3-week-old rat cortical cultures.
      Wang, Y; Zyskind, JW; Colacurcio, DJ; Lindl, KA; Ting, JH; Grigoriev, G; Jordan-Sciutto, KL
      Neuroreport  23  1052-8  2012

      Show Abstract
      23111339 23111339
      Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
      Suffert, G; Malterer, G; Hausser, J; Viiliäinen, J; Fender, A; Contrant, M; Ivacevic, T; Benes, V; Gros, F; Voinnet, O; Zavolan, M; Ojala, PM; Haas, JG; Pfeffer, S
      PLoS pathogens  7  e1002405  2011

      Show Abstract
      Western Blotting22174674 22174674
      Overexpression of miR-128 specifically inhibits the truncated isoform of NTRK3 and upregulates BCL2 in SH-SY5Y neuroblastoma cells.
      Guidi, M; Muiños-Gimeno, M; Kagerbauer, B; Martí, E; Estivill, X; Espinosa-Parrilla, Y
      BMC molecular biology  11  95  2010

      Show Abstract Full Text Article
      21143953 21143953
      Viral induction of central nervous system innate immune responses.
      Rempel, JD; Quina, LA; Blakely-Gonzales, PK; Buchmeier, MJ; Gruol, DL
      Journal of virology  79  4369-81  2005

      Show Abstract
      15767437 15767437
      Antiandrogen-induced cell death in LNCaP human prostate cancer cells.
      Lee, EC; Zhan, P; Schallhom, R; Packman, K; Tenniswood, M
      Cell death and differentiation  10  761-71  2003

      Show Abstract
      12815459 12815459
      Role of p53 and ATM in photodynamic therapy-induced apoptosis.
      Viola Heinzelmann-Schwarz, André Fedier, René Hornung, Heinrich Walt, Urs Haller, Daniel Fink
      Lasers in surgery and medicine  33  182-9  2003

      Show Abstract
      12949948 12949948

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      Included Positive Control

      Catalog Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies