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MAB3507 Anti-Caspase 2 Antibody, clone 11B4

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MAB3507
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, MkELISA, FC, IP, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3507
      Replaces04-571
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 2 Antibody, clone 11B4
      Alternate Names
      • ICH-1
      References
      Product Information
      FormatPurified
      PresentationLiquid with 0.05% Bronidox as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Caspase 2 Antibody, clone 11B4 is an antibody against Caspase 2 for use in ELISA, FC, IP & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.

      Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.

      EIA: Recommended concentration 0.5-1 μg/ml.

      Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRaised against the P19 subunit of recombinant human Caspase-2 protein.
      Clone11B4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityMAB3507 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2).
      IsotypeIgG2a
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
      Gene Symbol
      • CASP2
      • NEDD2
      • ICH1
      • NEDD-2
      • ICH-1L/1S
      • CASP-2
      • ICH-1L
      • EC 3.4.22.55 [Contains: Caspase-2 subunit p18
      • Caspase-2 subunit p13
      • Caspase-2 subunit p12].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
      SIZE: 452 amino acids; 50685 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.
      TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle.
      PTM: The mature protease can process its own propeptide, but not that of other caspases.
      SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB3507 04053252661969

      Documentation

      Anti-Caspase 2 Antibody, clone 11B4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase 2 Antibody, clone 11B4 Certificates of Analysis

      TitleLot Number
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal (100ug) 3019048
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal (100ug) - 3384540 3384540
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal (100ug) - 3690676 3690676
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal (100ug) - ME8MN5401 ME8MN5401
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal (100ug) - ME8NN7305 ME8NN7305
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal (100ug) - ME9DN8937 ME9DN8937
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal (100ug) - ME9EN5644 ME9EN5644
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 11B4 Rat Monoclonal - 08071509143 08071509143
      MAB3507 - 08061500674 08061500674

      References

      Reference overviewApplicationPub Med ID
      Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death.
      Del Nagro, CJ; Choi, J; Xiao, Y; Rangell, L; Mohan, S; Pandita, A; Zha, J; Jackson, PK; O'Brien, T
      Cell cycle (Georgetown, Tex.)  13  303-14  2014

      Show Abstract
      24247149 24247149
      Caspase-2 maintains bone homeostasis by inducing apoptosis of oxidatively-damaged osteoclasts.
      Sharma, R; Callaway, D; Vanegas, D; Bendele, M; Lopez-Cruzan, M; Horn, D; Guda, T; Fajardo, R; Abboud-Werner, S; Herman, B
      PloS one  9  e93696  2014

      Show Abstract
      24691516 24691516
      Intrinsic caspase-8 activation mediates sensitization of erlotinib-resistant tumor cells to erlotinib/cell-cycle inhibitors combination treatment.
      Orzáez, M; Guevara, T; Sancho, M; Pérez-Payá, E
      Cell death & disease  3  e415  2012

      Show Abstract
      23096116 23096116
      Squamous cell carcinoma antigen 1 promotes caspase-8-mediated apoptosis in response to endoplasmic reticulum stress while inhibiting necrosis induced by lysosomal injury.
      Ullman, E; Pan, JA; Zong, WX
      Molecular and cellular biology  31  2902-19  2011

      Show Abstract
      21576355 21576355
      Inhibition of protein degradation induces apoptosis through a microtubule-associated protein 1 light chain 3-mediated activation of caspase-8 at intracellular membranes.
      Pan, JA; Ullman, E; Dou, Z; Zong, WX
      Molecular and cellular biology  31  3158-70  2011

      Show Abstract
      21628531 21628531
      ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress.
      Myers, K; Gagou, ME; Zuazua-Villar, P; Rodriguez, R; Meuth, M
      PLoS genetics  5  e1000324  2009

      Show Abstract Full Text Article
      19119425 19119425
      Specific caspase interactions and amplification are involved in selective neuronal vulnerability in Huntington's disease.
      Hermel, E; Gafni, J; Propp, SS; Leavitt, BR; Wellington, CL; Young, JE; Hackam, AS; Logvinova, AV; Peel, AL; Chen, SF; Hook, V; Singaraja, R; Krajewski, S; Goldsmith, PC; Ellerby, HM; Hayden, MR; Bredesen, DE; Ellerby, LM
      Cell death and differentiation  11  424-38  2004

      Show Abstract
      Western Blotting14713958 14713958
      Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis.
      Mancini, M, et al.
      J. Cell Biol., 149: 603-12 (2000)  2000

      Show Abstract
      10791974 10791974
      Prodomain-dependent nuclear localization of the caspase-2 (Nedd2) precursor. A novel function for a caspase prodomain.
      Colussi, P A, et al.
      J. Biol. Chem., 273: 24535-42 (1998)  1998

      Show Abstract
      9733748 9733748

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies