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MAB3501 Anti-Caspase 2 Antibody, clone 10C6

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, Mk, RELISA, FC, IP, WBRPurifiedMonoclonal Antibody
      Catalogue NumberMAB3501
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 2 Antibody, clone 10C6
      Alternate Names
      • ICH-1
      Product Information
      PresentationPurified rat monoclonal antibody in buffer containing PBS, 0.1% Kathon.
      Quality LevelMQ100
      ApplicationAnti-Caspase 2 Antibody, clone 10C6 detects level of Caspase 2 & has been published & validated for use in ELISA, FC, IP & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.

      Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.

      EIA: Recommended concentration 0.5-1 μg/ml.

      Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRaised against the P19 subunit of recombinant human Caspase-2 protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityMAB3501 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2).
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Monkey
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
      Gene Symbol
      • CASP2
      • NEDD2
      • ICH1
      • NEDD-2
      • ICH-1L/1S
      • CASP-2
      • ICH-1L
      • EC [Contains: Caspase-2 subunit p18
      • Caspase-2 subunit p13
      • Caspase-2 subunit p12].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
      SIZE: 452 amino acids; 50685 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.
      TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle.
      PTM: The mature protease can process its own propeptide, but not that of other caspases.
      SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      MAB3501 04053252660849


      Anti-Caspase 2 Antibody, clone 10C6 SDS


      Safety Data Sheet (SDS) 

      Anti-Caspase 2 Antibody, clone 10C6 Certificates of Analysis

      TitleLot Number
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 10C6 Rat Monoclonal (100ug) - ME9AN5605 ME9AN5605
      Caspase-2 (Hu,Ms,Mk,Can,Rt) 10C6 Rat Monoclonal (100ug) - ME9DN3172 ME9DN3172
      RAT ANTI-CASPASE-2 (ICH-1) - 3385791 3385791
      RAT ANTI-CASPASE-2 (ICH-1) - 3483599 3483599
      RAT ANTI-CASPASE-2 (ICH-1) - 4150395 4150395
      RAT ANTI-CASPASE-2 (ICH-1) -2789763 2789763
      RAT ANTI-CASPASE-2 (ICH-1) MONOCLONAL ANTIBODY - 2430538 2430538
      RAT ANTI-CASPASE-2 (ICH-1) MONOCLONAL ANTIBODY - 2297245 2297245


      Reference overviewApplicationPub Med ID
      BH3-only protein BIM mediates heat shock-induced apoptosis.
      Mahajan, IM; Chen, MD; Muro, I; Robertson, JD; Wright, CW; Bratton, SB
      PloS one  9  e84388  2014

      Show Abstract
      24427286 24427286
      An L-tyrosine derivative and PPARgamma agonist, GW7845, activates a multifaceted caspase cascade in bone marrow B cells.
      Schlezinger, JJ; Emberley, JK; Bissonnette, SL; Sherr, DH
      Toxicological sciences : an official journal of the Society of Toxicology  98  125-36  2007

      Show Abstract
      17400580 17400580
      Specific caspase interactions and amplification are involved in selective neuronal vulnerability in Huntington's disease.
      Hermel, E; Gafni, J; Propp, SS; Leavitt, BR; Wellington, CL; Young, JE; Hackam, AS; Logvinova, AV; Peel, AL; Chen, SF; Hook, V; Singaraja, R; Krajewski, S; Goldsmith, PC; Ellerby, HM; Hayden, MR; Bredesen, DE; Ellerby, LM
      Cell death and differentiation  11  424-38  2004

      Show Abstract
      Western Blotting14713958 14713958
      Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis.
      Mancini, M, et al.
      J. Cell Biol., 149: 603-12 (2000)  2000

      Show Abstract
      10791974 10791974
      Prodomain-dependent nuclear localization of the caspase-2 (Nedd2) precursor. A novel function for a caspase prodomain.
      Colussi, P A, et al.
      J. Biol. Chem., 273: 24535-42 (1998)  1998

      Show Abstract
      9733748 9733748

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      Life Science Research > Antibodies and Assays > Primary Antibodies