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AB1871 Anti-Caspase 1 Antibody

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AB1871
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIF, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1871
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 1 Antibody
      Alternate Names
      • ICE
      Background InformationCaspase-1 (ICE, interleukin-1b-converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of interleukin-1b and IL-18 into active cytokines. Like other caspases, caspase-1 itself is synthesized as a proenzyme that is cleaved during activation into large (20 kDa) and small (10 kDa) subunits.
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in PBS, pH 7.2, containing 50% glycerol, 1% BSA and 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Caspase 1 Antibody is validated for use in IF, IP, WB for the detection of Caspase 1.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 1-4 μg/mL

      Immunoprecipitation: 4-8 μg/mL

      Immunofluorescence: 10-20 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAB1871 recognizes the 45 kDa procaspase-1 as well as the 20 kDa cleaved caspase-1.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
      Gene Symbol
      • CASP1
      • IL1BC
      • p45
      • ICE
      • IL1BCE
      • P45
      • IL-1BC
      • IL1B-convertase
      • CASP-1
      • EC 3.4.22.36 [Contains: Caspase-1 subunit p20
      • Caspase-1 subunit p10].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
      SIZE: 404 amino acids; 45159 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
      PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
      SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C. For optimal storage, aliquot and freeze at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB1871 04053252278990

      Documentation

      Anti-Caspase 1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase 1 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-CASPASE-1 [ICE] - 2488805 2488805
      RABBIT ANTI-CASPASE-1 [ICE] - 3202395 3202395
      RABBIT ANTI-CASPASE-1 [ICE] - 3205442 3205442
      RABBIT ANTI-CASPASE-1 [ICE] - 3231902 3231902
      RABBIT ANTI-CASPASE-1 [ICE] - 3290438 3290438
      RABBIT ANTI-CASPASE-1 [ICE] - 3440722 3440722
      RABBIT ANTI-CASPASE-1 [ICE] - 3499354 3499354
      RABBIT ANTI-CASPASE-1 [ICE] - 3587202 3587202
      RABBIT ANTI-CASPASE-1 [ICE] - 3616512 3616512
      RABBIT ANTI-CASPASE-1 [ICE] - 3836555 3836555

      References

      Reference overviewPub Med ID
      Neurodegeneration and Vision Loss after Mild Blunt Trauma in the C57Bl/6 and DBA/2J Mouse.
      Bricker-Anthony, C; Rex, TS
      PloS one  10  e0131921  2015

      Show Abstract
      26148200 26148200
      HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-κB pathway in acute glaucoma.
      Chi, W; Chen, H; Li, F; Zhu, Y; Yin, W; Zhuo, Y
      Journal of neuroinflammation  12  137  2015

      Show Abstract
      26224068 26224068
      Adenovirus membrane penetration activates the NLRP3 inflammasome.
      Barlan, AU; Griffin, TM; McGuire, KA; Wiethoff, CM
      Journal of virology  85  146-55  2011

      Show Abstract
      20980503 20980503
      Expression of interleukin-18 is increased in the brains of Alzheimer's disease patients.
      Johanna Ojala,Irina Alafuzoff,Sanna-Kaisa Herukka,Thomas van Groen,Heikki Tanila,Tuula Pirttilä
      Neurobiology of aging  30  2009

      Show Abstract
      17658666 17658666
      Simultaneous human papilloma virus type 16 E7 and cdk inhibitor p21 expression induces apoptosis and cathepsin B activation.
      Kaznelson, DW; Bruun, S; Monrad, A; Gjerløv, S; Birk, J; Röpke, C; Norrild, B
      Virology  320  301-12  2004

      Show Abstract
      15016552 15016552

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies