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PC205L Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

PC205L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
M, R Rb Polyclonal Antibody

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PC205L-100UL
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      Description
      OverviewRecognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.
      Catalogue NumberPC205L
      Brand Family Calbiochem®
      SynonymsAnti-CGRP
      Application Data
      Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
      References
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat amygdala or spinal cord
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections, Free Floating Sections, Immunofluorescence Maatkamp, A., et al. 2004. Eur. J. Neurosci. 20, 14.
      Key Applications Paraffin Sections
      Free-floating Sections
      Frozen Sections
      Immunofluorescence
      Application NotesFree Floating Sections (see application references)
      Frozen Sections (1:2000-1:4000, ABC detection)
      Immunofluorescence (1:100-1:200, see application references)
      Paraffin Sections (see application references; heat pre-treatment required; see comments)
      Application CommentsAntibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrat α-calcitonin gene related peptide (α-CGRP)
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC205L

      References

      Reference overview
      Sudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      SynonymsAnti-CGRP
      ApplicationFree Floating Sections (see application references)
      Frozen Sections (1:2000-1:4000, ABC detection)
      Immunofluorescence (1:100-1:200, see application references)
      Paraffin Sections (see application references; heat pre-treatment required; see comments)
      Application Data
      Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
      BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The calcitonin gene peptide superfamily consists of calcitonin, calcitonin gene-related peptide (CGRP), amylin, and adrenomedullin. CGRP is a 37 amino acid neuropeptide that shows widespread distribution and acts as a potent vasodilator. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
      HostRabbit
      Immunogen speciesRat
      Immunogenrat α-calcitonin gene related peptide (α-CGRP)
      IsotypeIgG
      Speciesmouse, rat
      Positive controlRat amygdala or spinal cord
      FormLyophilized
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Application referencesParaffin Sections, Free Floating Sections, Immunofluorescence Maatkamp, A., et al. 2004. Eur. J. Neurosci. 20, 14.