681507 Anti-CaV1.2 (818-835) Rabbit pAb

681507
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
M, Rb, R Rb Polyclonal Antibody

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      Description
      OverviewRecognizes the ~190 kDa and ~210 forms of the α1C-subunit. Supplied with a control peptide. Note: The α-subunit is highly sensitive to proteases. Please refer to the data sheet for proper sample preparation.
      Catalogue Number681507
      Brand Family Calbiochem®
      SynonymsAnti-L-Type Ca2+ Channel
      Application Data
      Detection of rat CaV1.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:200). Detection: chemiluminescence.

      Detection of mouse CaV1.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV1.2 channel (red) appearing in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) in the section demonstrates the location of dendrites in the molecular layer (Figure B). Confocal merge of CaV1.2 and CBD28K (Figure C). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:100). Detection: fluorescence.
      References
      ReferencesHell, J.W., et al. 1995. EMBO J. 14, 3036.
      Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
      Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
      Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
      Snutch, T.P. et al. 1991. Neuron 7, 45.
      Product Information
      FormLyophilized
      Formulation50 µl antibody in PBS, 5% sucrose, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
      Positive controlRat brain or heart
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunocytochemistry Hell, J.W., et al. 1995. EMBO J. 14, 3036. Immunoprecipitation Hell, J.W., et al. 1995. EMBO J. 14, 3036. Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
      Key Applications Immunoprecipitation
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunohistochemistry
      Application NotesImmunoblotting (1:200)
      Immunocytochemistry (see application references)
      Immunoprecipitation (see application references)
      Immunohistochemistry (!:100)
      Application CommentsFor use as a negative control, preincubate 1 µg of CaV1.2 control peptide with 1 µg of antibody for 1 h at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [(C)TTKINMDDLQPSENEDKS] corresponding to amino acids 848-865 of α1C-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rabbit
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDilutions of the antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the CaV1.2 control peptide with 100 µl deionized H₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of antibody and peptide solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10,000 x g for 5 min) to remove any aggregates.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CaV1.2 (818-835) Rabbit pAb Certificates of Analysis

      TitleLot Number
      681507

      References

      Reference overview
      Hell, J.W., et al. 1995. EMBO J. 14, 3036.
      Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
      Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
      Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
      Snutch, T.P. et al. 1991. Neuron 7, 45.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2011 JSW
      SynonymsAnti-L-Type Ca2+ Channel
      ApplicationImmunoblotting (1:200)
      Immunocytochemistry (see application references)
      Immunoprecipitation (see application references)
      Immunohistochemistry (!:100)
      Application Data
      Detection of rat CaV1.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:200). Detection: chemiluminescence.

      Detection of mouse CaV1.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV1.2 channel (red) appearing in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) in the section demonstrates the location of dendrites in the molecular layer (Figure B). Confocal merge of CaV1.2 and CBD28K (Figure C). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:100). Detection: fluorescence.
      DescriptionImmunoaffinity purified Rabbit polyclonal antibody. Recognizes the ~190 kDa and the ~210 kDa forms of the α1C-subunit of the voltage-gated Ca2+ channel. Supplied with a control peptide (immunogen).
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide [(C)TTKINMDDLQPSENEDKS] corresponding to amino acids 848-865 of α1C-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
      IsotypeIgG
      Speciesmouse, rabbit, rat
      Positive controlRat brain or heart
      FormLyophilized
      Formulation50 µl antibody in PBS, 5% sucrose, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
      PreservativeNone
      CommentsFor use as a negative control, preincubate 1 µg of CaV1.2 control peptide with 1 µg of antibody for 1 h at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDilutions of the antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the CaV1.2 control peptide with 100 µl deionized H₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of antibody and peptide solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10,000 x g for 5 min) to remove any aggregates.
      Toxicity Standard Handling
      ReferencesHell, J.W., et al. 1995. EMBO J. 14, 3036.
      Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
      Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
      Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
      Snutch, T.P. et al. 1991. Neuron 7, 45.
      Application referencesImmunocytochemistry Hell, J.W., et al. 1995. EMBO J. 14, 3036. Immunoprecipitation Hell, J.W., et al. 1995. EMBO J. 14, 3036. Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.