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AP1135 Anti-CTNNBL1 Mouse mAb (5F1)

AP1135
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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AP1135-50UG
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      Glass bottle 50 μg
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      Description
      OverviewRecognizes the ~65-70 kDa CTNNBL1 protein in HeLa cells and human testes tissue.
      Catalogue NumberAP1135
      Brand Family Calbiochem®
      SynonymsAnti-Beta catenin like protein 1
      Application Data
      Detection of human CTNNBL1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CTNNBL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (40 µg/ml). Detection: fluorescence.

      Detection of humsn CTNNBL1 by paraffin sections. Sample: Formalin-fixed paraffin-embedded human testis. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2
      Positive controlHeLa cells, testes tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (40 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA synthtic polypeptide corresponding to amino acids 210-311 of human rCTNNBL1
      ImmunogenNA
      Clone5F1
      HostMouse
      IsotypeIgG3
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial, thaw aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CTNNBL1 Mouse mAb (5F1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CTNNBL1 Mouse mAb (5F1) Certificates of Analysis

      TitleLot Number
      AP1135
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-October-2010 JLN
      SynonymsAnti-Beta catenin like protein 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (40 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human CTNNBL1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CTNNBL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (40 µg/ml). Detection: fluorescence.

      Detection of humsn CTNNBL1 by paraffin sections. Sample: Formalin-fixed paraffin-embedded human testis. Primary antibody: Anti-CTNNBL1 Mouse mAb (5F1) (Cat. No. AP1135) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~65-70 kDa CTNNBL1 protein.
      BackgroundCTNNBL1 (Beta-catenin-like protein 1) is an apoptosis inducing protein with a structural homology similar to that of beta-catenin. CTNNBL1 is highly conservered across various species and it is primarily expressed in the nucleus of various tissues. In human, CTNNBL1 shows highest expression in skeletal muscle, placenta, heart, spleen, testis, and thyroid tissues. CTNNBL1 has been linked to a possible role in the Wnt/beta-catenin-signaling pathway and may have a function similar to that of beta catenin.
      HostMouse
      Immunogen speciesNA
      ImmunogenA synthtic polypeptide corresponding to amino acids 210-311 of human rCTNNBL1
      Clone5F1
      IsotypeIgG3
      Specieshuman
      Positive controlHeLa cells, testes tissue
      Formliquid
      FormulationIn PBS, pH 7.2
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial, thaw aliquot and freeze (-20°C).
      Toxicity Regulatory Review