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CB1046 Anti-CLDN1 Mouse mAb (1C5-D9)

This Anti-CLDN1 Mouse mAb (1C5-D9) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of CLDN1.

Anti-CLDN1 Mouse mAb (1C5-D9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Claudin 1

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CB1046
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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CB1046-100UG
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      Description
      OverviewRecognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.
      Catalogue NumberCB1046
      Brand Family Calbiochem®
      SynonymsAnti-Claudin 1
      Application Data
      Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from PRC/PRF/5 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (5 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesGuevin C, et al. 2009. Antiviral Res. 84, 14.
      Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
      Stamataki Z, et al. 2009. Blood 113 585.
      Farquhar MJ, et al. 2008. J. Virol. 82 8797.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlPRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
      Clone1C5-D9
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Identification Number
      Catalog Number GTIN
      CB1046-100UG4055977228410

      Documentation

      Anti-CLDN1 Mouse mAb (1C5-D9) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Guevin C, et al. 2009. Antiviral Res. 84, 14.
      Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
      Stamataki Z, et al. 2009. Blood 113 585.
      Farquhar MJ, et al. 2008. J. Virol. 82 8797.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Claudin 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3-5 µg/ml)
      Application Data
      Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from PRC/PRF/5 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (5 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.
      BackgroundCLDN1 is a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations results in neonatal ichthyosis-sclerosing cholangitis syndrome.
      HostMouse
      ImmunogenFull-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
      Clone1C5-D9
      IsotypeIgG2a
      Specieshuman
      Positive controlPRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesGuevin C, et al. 2009. Antiviral Res. 84, 14.
      Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
      Stamataki Z, et al. 2009. Blood 113 585.
      Farquhar MJ, et al. 2008. J. Virol. 82 8797.