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NE1004 Anti-Apolipoprotein E Mouse mAb (E6D7)

NE1004
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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NE1004-100UL
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      Description
      OverviewRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.
      Catalogue NumberNE1004
      Brand Family Calbiochem®
      SynonymsAnti-ApoE
      Application Data
      Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence.

      Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB.

      Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
      References
      ReferencesGolabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.
      Product Information
      FormLiquid
      FormulationCulture supernatant diluted in PBS.
      Positive controlBrain tissue, serum
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Paraffin Sections
      Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1:100-1:1000)
      Immunoprecipitation (see comments)
      Frozen Sections (1:100-1:10,000)
      Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
      Application CommentsRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
      ImmunogenHuman
      CloneE6D7
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Apolipoprotein E Mouse mAb (E6D7) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Apolipoprotein E Mouse mAb (E6D7) Certificates of Analysis

      TitleLot Number
      NE1004

      References

      Reference overview
      Golabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-October-2010 JSW
      SynonymsAnti-ApoE
      ApplicationELISA (see comments)
      Immunoblotting (1:100-1:1000)
      Immunoprecipitation (see comments)
      Frozen Sections (1:100-1:10,000)
      Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
      Application Data
      Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence.

      Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB.

      Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
      DescriptionMouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.
      BackgroundApolipoprotein E (Apo E) is a polymorphic, multifunctional protein synthesized by liver, kidney, brain, and other cell types. It has been reported that the genetic inheritance of apolipoprotein isoform E4 is a significant risk factor for late onset Alzheimer's Disease (AD).
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
      CloneE6D7
      IsotypeIgG₁
      Specieshuman, not mouse, not rabbit
      Positive controlBrain tissue, serum
      FormLiquid
      FormulationCulture supernatant diluted in PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGolabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.