Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|Presentation||Rabbit antiserum. Liquid with 0.05% sodium azide.|
|Safety Information according to GHS|
|Material Size||100 µL|
Anti-Acetylcholine Antibody SDS
|Reference overview||Pub Med ID|
|Neurotransmitter synthesis in poststroke cortical neurogenesis in adult rats.|
Gu, W; Gu, C; Jiang, W; Wester, P
Stem cell research 4 148-54 2010
Neurogenesis occurs in the cerebral cortex of adult rats after focal cerebral ischemia. Whether or not the newborn neurons could synthesize neurotransmitters is unknown. To elucidate such a possibility, a photothrombotic ring stroke model with spontaneous reperfusion was induced in adult male Wistar rats. The DNA duplication marker BrdU was repeatedly injected, and the rats were sacrificed at various times after stroke. To detect BrdU nuclear incorporation and various neurotransmitters, brain sections were processed for single/double immunocytochemistry and single/double/triple immunofluorescence. Stereological cell counting was performed to assess the final cell populations. At 48 h, 5 days, 7 days, 30 days, 60 days and 90 days after stroke, numerous cells were BrdU-immunolabeled in the penumbral cortex. Some of these were doubly immunopositive to the cholinergic neuron-specific marker ChAT or GABAergic neuron-specific marker GAD. As analyzed by 3-D confocal microscopy, the neurotransmitters acetylcholine and GABA were colocalized with BrdU in the same cortical cells. In addition, GABA was colocalized with the neuron-specific marker Neu N in the BrdU triple-immunolabeled cortical cells. This study suggests that the newborn neurons are capable of synthesizing the neurotransmitters acetylcholine and GABA in the penumbral cortex, which is one of the fundamental requisites for these neurons to function in the poststroke recovery.
|Immunocytochemical studies of the distribution of acetylcholine in the crayfish brain.|
Wang-Bennett, L T, et al.
J. Comp. Neurol., 273: 330-43 (1988) 1988
A number of studies indicate that acetylcholine is an important transmitter in most crustacean primary afferents and in at least several central pathways. Little is known, however, regarding the structure or distribution of cholinergic pathways in the central nervous system. The recent introduction of antibodies to choline-protein conjugates provides a potentially powerful means for localizing putative cholinergic neurons and pathways in the nervous system. Acetylcholine was localized with immunocytochemical procedures in the axons and terminals of cephalic primary afferents and in interneurons of the crayfish brain. The most intensely reactive loci were the primary sensory neuropiles, which contain the terminals of the statocyst afferents (parolfactory lobes) and antennal afferents (antennal lobe). These results are generally in accord with previous findings based upon choline uptake and enzyme assay in lobster cephalic nerves. We also found evidence consistent with the presence of acetylcholine in the globular interneurons of the accessory lobe and in descending interneurons which originate in the dorsal medial and anterior clusters of the protocerebrum. The axons of several neurons in the circumesophageal connective (descending interneurons and primary afferents) are also reactive to the choline antibody.
|One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain.|
McRae-Degueurce, A and Geffard, M
Brain Res., 376: 217-9 (1986) 1986
A perfusion mixture composed of glutaraldehyde and allyl-alcohol at pH 12 is compatible for the fixation and immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rodent central nervous system. A technical description of this procedure is provided to facilitate the replication of this immunocytochemical approach with these antibodies.
|Anti-acetylcholine antibodies and first immunocytochemical application in insect brain.|
Geffard, M, et al.
Neurosci. Lett., 57: 1-6 (1985) 1985
A specific immunological approach was developed to enable acetylcholine (ACh) to be visualized in biological tissues. A variety of ACh-like immunogens were synthesized, and injected into rabbits. Antibody specificity was tested using an enzyme-linked immunosorbent assay (ELISA) method. The most immunoreactive ACh derivative was found to be choline-glutaryl-lysine. A mixture of allyl alcohol and formaldehyde was found to be the best fixative of ACh in tissues. The specificity of this antibody recognition was tested in vitro and in immunochemistry. There was excellent agreement between the in vitro results and the ACh staining. Moreover, visualization using these anti-ACh antibodies appeared identical to the results using anti-choline acetyltransferase antibodies.