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OP80 Anti-APC (Ab-7) Mouse mAb (CC-1)

OP80
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

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OP80-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes APC in oligodendrocytes and astrocytes.
    • Antibody Target Gene Symbol: APC
    • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
    • Entrez Gene Name: adenomatous polyposis coli
    • Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
    • Mu Entrez ID: 11789
    • Rat Entrez ID: 24205
    • Catalogue NumberOP80
      Brand Family Calbiochem®
      SynonymsAnti-Adenomatus Polyposis Coli
      References
      ReferencesBhat, R.V., et al. 1996. Glia 17, 169.
      Bhat, R.V., et al. 1994. Neuroscience Proceedings.
      Smith, K.J., et al. 1994. Cancer Res. 54, 3672.
      Smith, K.J., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2846.
      Su, L.-K., et al. 1993. Cancer Res. 53, 2728.
      Su, L.-K., et al. 1993. Science 262, 1734.
      Groden, J., et al. 1991. Cell 66, 589.
      Kinzler, K.W., et al. 1991. Science 251, 1366.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlMOPC 21 cells
      Positive controlPurkinje cells or cerebellum tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunocytochemistry McTigue, D.M., et al. 2001. J. Neurosci. 21, 3392. Free Floating Sections Ding, H., et al. 2003. Cancer Res. 63, 1106.
      Key Applications Immunofluorescence
      Paraffin Sections
      Free-floating Sections
      Frozen Sections
      Immunocytochemistry
      Application NotesFrozen Sections (5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Paraffin Sections (5 µg/ml, heat pre-treament required)
      Immunocytochemistry (see application references)
      Free Floating Sections (see application references)
      Application CommentsWell suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody's staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-226 of APC
      CloneCC-1
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-APC (Ab-7) Mouse mAb (CC-1) Certificates of Analysis

      TitleLot Number
      OP80

      References

      Reference overview
      Bhat, R.V., et al. 1996. Glia 17, 169.
      Bhat, R.V., et al. 1994. Neuroscience Proceedings.
      Smith, K.J., et al. 1994. Cancer Res. 54, 3672.
      Smith, K.J., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2846.
      Su, L.-K., et al. 1993. Cancer Res. 53, 2728.
      Su, L.-K., et al. 1993. Science 262, 1734.
      Groden, J., et al. 1991. Cell 66, 589.
      Kinzler, K.W., et al. 1991. Science 251, 1366.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-November-2007 JSW
      SynonymsAnti-Adenomatus Polyposis Coli
      ApplicationFrozen Sections (5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Paraffin Sections (5 µg/ml, heat pre-treament required)
      Immunocytochemistry (see application references)
      Free Floating Sections (see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing CB6 F1 mice with the specified immunogen and fusing splonocytes with SP2/O mouse myeloma cells. Recognizes the ~312 kDa APC protein.
      BackgroundGerm line mutations in the APC gene are responsible for familial adenomatous polyposis and somatic mutations contribute to sporadic colorectal cancer. The 312 kDa APC protein is expressed in all tissues and localized to the cytoskeletal architecture in an insoluble complex with β-catenin. The APC (Ab-7) mouse monoclonal antibody clone CC-1 decorates oligodendrocyte cell bodies, purkinje cells, astrocytes, and optic nerve cell bodies of formalin-fixed, paraffin-embedded, or fresh frozen sections from human, mouse, and rat brain. Dectection of APC (Ab-7) can be perfomed using immunofluorescence or immunohistochemistry techniques.
      HostMouse
      Immunogena recombinant protein consisting of amino acids 1-226 of APC
      CloneCC-1
      IsotypeIgG2b
      Specieshuman, mouse, rat
      Positive controlPurkinje cells or cerebellum tissue
      Negative controlMOPC 21 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsWell suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody's staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBhat, R.V., et al. 1996. Glia 17, 169.
      Bhat, R.V., et al. 1994. Neuroscience Proceedings.
      Smith, K.J., et al. 1994. Cancer Res. 54, 3672.
      Smith, K.J., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2846.
      Su, L.-K., et al. 1993. Cancer Res. 53, 2728.
      Su, L.-K., et al. 1993. Science 262, 1734.
      Groden, J., et al. 1991. Cell 66, 589.
      Kinzler, K.W., et al. 1991. Science 251, 1366.
      Application referencesImmunocytochemistry McTigue, D.M., et al. 2001. J. Neurosci. 21, 3392. Free Floating Sections Ding, H., et al. 2003. Cancer Res. 63, 1106.