ST1507 Anti-AKR1B10 Mouse mAb (1A6)

ST1507
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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ST1507-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
      Catalogue NumberST1507
      Brand Family Calbiochem®
      SynonymsAnti-Aldo-Keto Reductase 1B10
      Application Data
      Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human AKR1B10 by immunocytochemistry. Sample: HeLa cellsfixed in Paraformaldehyde. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesAhn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
      Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHepG2 cells, Human Colon tissue, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
      ImmunogenNA
      Clone1A6
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-AKR1B10 Mouse mAb (1A6) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
      Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Aldo-Keto Reductase 1B10
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human AKR1B10 by immunocytochemistry. Sample: HeLa cellsfixed in Paraformaldehyde. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.
      BackgroundThis gene product encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis.
      HostMouse
      Immunogen speciesNA
      ImmunogenA recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
      Clone1A6
      IsotypeIgG2a
      Specieshuman
      Positive controlHepG2 cells, Human Colon tissue, HeLa cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesAhn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
      Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.