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ABS988 Anti-ACAT-2 Antibody

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ABS988
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MkWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS988
      DescriptionAnti-ACAT-2 Antibody
      Alternate Names
      • SOAT2_HUMAN
      • SOAT2
      • ACACT2
      • ACTL
      Background InformationAcyl-coenzyme A:cholesterol acyltransferase 2 (ACAT-2), also known as Sterol O-acyltransferase 2
      (SOAT2), is a synthetic enzyme that is important in lipoprotein assembly, steroid metabolism, and dietary cholesterol metabolism and absorption. ACAT-2 is expressed primarily in the liver and intestine in enterocytes in their endoplasmic reticulum. ACAT-2 esterifies cholesterol allowing it to be more efficiently packaged and transported. Free cholesterol can be taken up by lipoproteins but it is confined to the outer surface of the lipoparticle. Esterification allows internal packaging and much more efficient transport through the blood stream. Gene expression of ACAT-2 is partially regulated by the transcriptional repressor TGIF1 as well as other factors such as thyroid hormone expression and microRNA miR181d.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit Polyclonal in buffer containing 20 mM NaPO4, 28 mM NaCl and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-ACAT-2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ACAT-2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected ACAT-2 in human liver tissue (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023).
      Western Blot Analysis: A representative lot detected ACAT-2 in human liver lysate (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023)
      Biological Information
      ImmunogenRecombinant protein corresponding to Human ACAT-2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Monkey
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SOAT2
      • ACACT2
      • ACAT2
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~ 55 kDa observed, uncharacterized bands may appear at lower molecular weight
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human lung tissue lysate using a non-reduced gel.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ACAT-2 in 10 µg of human lung tissue lysate using a non-reduced gel
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS988 04053252993107

      Documentation

      Anti-ACAT-2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ACAT-2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-ACAT-2 - Q2452355 Q2452355
      Anti-ACAT-2 - 3389902 3389902
      Anti-ACAT-2 - 4035056 4035056

      References

      Reference overviewPub Med ID
      TG-interacting factor 1 acts as a transcriptional repressor of sterol O-acyltransferase 2.
      Pramfalk, C; Melhuish, TA; Wotton, D; Jiang, ZY; Eriksson, M; Parini, P
      Journal of lipid research  55  709-17  2014

      Show Abstract
      24478032 24478032
      Thyroid hormone negatively regulates CDX2 and SOAT2 mRNA expression via induction of miRNA-181d in hepatic cells.
      Yap, CS; Sinha, RA; Ota, S; Katsuki, M; Yen, PM
      Biochemical and biophysical research communications  440  635-9  2013

      Show Abstract
      24103759 24103759
      Tissue-specific knockouts of ACAT2 reveal that intestinal depletion is sufficient to prevent diet-induced cholesterol accumulation in the liver and blood.
      Zhang, Jun, et al.
      J. Lipid Res., 53: 1144-52 (2012)  2012

      Show Abstract
      22460046 22460046
      ACAT2 and human hepatic cholesterol metabolism: identification of important gender-related differences in normolipidemic, non-obese Chinese patients.
      Parini, Paolo, et al.
      Atherosclerosis, 207: 266-71 (2009)  2009

      Show Abstract
      19467657 19467657
      Identification of the interaction site within acyl-CoA:cholesterol acyltransferase 2 for the isoform-specific inhibitor pyripyropene A.
      Das, Akash, et al.
      J. Biol. Chem., 283: 10453-60 (2008)  2008

      Show Abstract
      18285335 18285335
      Identification of putative active site residues of ACAT enzymes.
      Das, Akash, et al.
      J. Lipid Res., 49: 1770-81 (2008)  2008

      Show Abstract
      18480028 18480028
      ACAT2 is localized to hepatocytes and is the major cholesterol-esterifying enzyme in human liver.
      Parini, Paolo, et al.
      Circulation, 110: 2017-23 (2004)  2004

      Show Abstract
      15451793 15451793
      Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates.
      Lee, R G, et al.
      J. Lipid Res., 41: 1991-2001 (2000)  2000

      Show Abstract
      11108732 11108732