NA02A Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

NA02A
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

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NA02A-0.5ML
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      Description
      OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
      Catalogue NumberNA02A
      Brand Family Calbiochem®
      References
      ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
      Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Product Information
      FormLiquid suspension
      FormulationIn PBS.
      Negative controlTransfer RNA (tRNA)
      Positive controlHeterogenous nuclear RNA (hnRNA)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.
      Key Applications Affinity Purification
      Immunoblotting (Western Blotting)
      Application NotesAffinity Purification (see application references)
      Immunoprecipitation (15 µl beads/sample)
      Immunoprecipitation (1-2 µg/ml; see application references)
      Immunocytochemistry (use Cat. No. NA02)
      Paraffin Sections (use Cat. No. NA02)
      Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogen2,2,7-trimethylguanosine
      CloneK121
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate Certificates of Analysis

      TitleLot Number
      NA02A

      References

      Reference overview
      Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
      Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      ApplicationAffinity Purification (see application references)
      Immunoprecipitation (15 µl beads/sample)
      Immunoprecipitation (1-2 µg/ml; see application references)
      Immunocytochemistry (use Cat. No. NA02)
      Paraffin Sections (use Cat. No. NA02)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5' cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
      BackgroundThe U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
      HostMouse
      Immunogen2,2,7-trimethylguanosine
      CloneK121
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlHeterogenous nuclear RNA (hnRNA)
      Negative controlTransfer RNA (tRNA)
      FormLiquid suspension
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
      Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Application referencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies