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AB9750 Anti-14-3-3 phospho Serine58 Antibody

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AB9750
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9750
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-14-3-3 phospho Serine58 Antibody
      References
      Product Information
      FormatAffinity Purified
      PresentationLiquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-14-3-3 phospho Serine58 Antibody detects level of 14-3-3 phospho Serine58 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:1,000
      Biological Information
      ImmunogenSynthetic peptide of amino acids surrounding the phosphoSerine 58 site of rat 14-3-3 Protein.
      HostRabbit
      Specificity14-3-3 Protein, phosphoSerine58. The antibody recognizes a protein of ~29 kDa corresponding to 14-3-3 Protein, phosphoSerine58 in lysates from rat brainstem. Immunolabeling is blocked by the phosphopeptide used as the immunogen but not the corresponding non-phosphopeptide.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteThe immunogen sequence has 100% conservation with mouse and Xenopus.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
      Gene Symbol
      • YWHAE
      • KCIP-1
      • MDS
      • 14-3-3E
      • MDCR
      • FLJ45465
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
      SIZE: 255 amino acids; 29174 Da
      SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB9750 04053252675447

      Documentation

      Anti-14-3-3 phospho Serine58 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-14-3-3 phospho Serine58 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-14-3-3 PROTEIN, phosphoSerine58 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2903913
      RABBIT ANTI-14-3-3 PROTEIN, phosphoSerine58 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2152451 2152451
      RABBIT ANTI-14-3-3 PROTEIN, phosphoSerine58 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2049255 2049255
      RABBIT ANTI-14-3-3 PROTEIN, phosphoSerine58 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2196033 2196033
      RABBIT ANTI-14-3-3 PROTEIN, phosphoSerine58 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2197193 2197193

      References

      Reference overviewPub Med ID
      14-3-3 proteins: a number of functions for a numbered protein
      Bridges, D. and Moorhead, G.B.G.
      Sci STKE, 2005:re10 (2005)  2005

      16091624 16091624
      14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding
      Silhan, J., et al.
      J. Biol. Chem., 279:49113-49119 (2004)  2004

      15347690 15347690
      Unlocking the code of 14-3-3.
      Dougherty, Michele K and Morrison, Deborah K
      J. Cell. Sci., 117: 1875-84 (2004)  2004

      Show Abstract
      15090593 15090593
      14-3-3 proteins in the nervous system
      Berg, Daniela, et al
      Nat Rev Neurosci, 4:752-62 (2003)  2003

      12951567 12951567
      The dimeric versus monomeric status of 14-3-3zeta is controlled by phosphorylation of Ser58 at the dimer interface
      Woodcock, J. M., et al.
      J. Biol. Chem., 278:36323-36327 (2003)  2003

      12865427 12865427

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies